We narrowed to 69 results for: gja1

1. pGL4.23-Gja1prom

   Plasmid

   #188114

   Purpose

   Expresses luciferase under control of Gja1 promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

   Insert

   Gja1 promoter (Gja1 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Gja1 endogenous promoter up to gene start codon

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-43: GJA1-mEGFP

   Plasmid

   #109120

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

   Depositor

   Allen Institute for Cell Science

   Insert

   GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   May 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pcDNA3.2-GJA1-S373A

   Plasmid

   #52879

   Purpose

   Encodes human Connexin 43 resistant to siRNA with Serine to Alanine mutation at 373

   Depositor

   Robin Shaw

   Article

   Smyth et al Traffic. 2014 Mar 10. doi: 10.1111/tra.1

   Insert

   GJA1 (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Serine 373 mutated to Alalnine

   Promoter

   CMV

   Available since

   May 12, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.2-GJA1-20K-V5

   Plasmid

   #49859

   Purpose

   Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   GJA1-20k (GJA1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   GJA1-20k fragment corresponds to residues 213-382…

   Promoter

   CMV

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pDEST-mCherry-GJA1-20k-N1

   Plasmid

   #49861

   Purpose

   Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   GJA1-20k (GJA1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   GJA1-20k corresponds to residues 213 to 382 to Co…

   Promoter

   CMV

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cx43-msfGFP

   Plasmid

   #69007

   Purpose

   Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Gja1 (Gja1 Rat)
   

   Use

   Tags

   monomerized superfolder GFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. msfGFP-Cx43

   Plasmid

   #69024

   Purpose

   Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSFFV-Cx43

   Plasmid

   #69442

   Purpose

   mammalian expression of rat Cx43

   Depositor

   Richard Veenstra

   Article

   Veenstra et al Circ Res. 1995 Dec;77(6):1156-65.

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pBS-Cx43

    Plasmid

    #65439

    Purpose

    Cloning, T7 RNA expression

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    T7 in vitro rna production

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPCX-Cx43-IRES-GFP

    Plasmid

    #65433

    Purpose

    Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cx43K258stop-msfGFP

    Plasmid

    #69023

    Purpose

    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    V206K monomerized super folder GFP

    Expression

    Mammalian

    Mutation

    delete codons 258-381 and fuse in frame to monome…

    Promoter

    CMV

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.2-Cx43-siResist

    Plasmid

    #49852

    Purpose

    Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains several wobble mutations to confer resis…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

14. Cx43-sfGFP

    Plasmid

    #70053

    Purpose

    Expresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    non-monomerized superfolder GFP (V207)

    Expression

    Mutation

    fused in frame to non-monomerized superfolder GFP

    Promoter

    CMV

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EBFP2-Cx43

    Plasmid

    #69020

    Purpose

    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    EBFP2

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS-Cx43-FS

    Plasmid

    #65440

    Purpose

    Cloning, T7 RNA expression

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    T7 in vitro rna production

    Tags

    Expression

    Bacterial

    Mutation

    Frameshift (FS) mutation 35delA

    Promoter

    T7

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.2-Cx43-HA

    Plasmid

    #49851

    Purpose

    Encodes human connexin 43 with a C-terminal HA epitope fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains several wobble mutations to confer resis…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLPCX-Cx43-FS-IRES-GFP

    Plasmid

    #65438

    Purpose

    Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    frameshift (FS) mutation 35delA

    Promoter

    CMV

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sfGFP-Cx43K258stop

    Plasmid

    #69025

    Purpose

    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    super folder GFP

    Expression

    Mammalian

    Mutation

    non-monomerized sfGFP fused in frame to N-terminu…

    Promoter

    CMV

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST-EGFP-Cx43ML-N1

    Plasmid

    #49860

    Purpose

    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    All internal Methionines mutated to Leucine. No s…

    Promoter

    CMV

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only