Showing: 1 - 20 of 47 results
-
Plasmid#188114PurposeExpresses luciferase under control of Gja1 promoter in transfected cellsDepositorInsertGja1 promoter (Gja1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterGja1 endogenous promoter up to gene start codonAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-GJA1-20K-V5
Plasmid#49859PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
UseTagsV5ExpressionMammalianMutationGJA1-20k fragment corresponds to residues 213-382…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-GJA1-S373A
Plasmid#52879PurposeEncodes human Connexin 43 resistant to siRNA with Serine to Alanine mutation at 373DepositorInsertGJA1 (GJA1 Human)
UseTagsExpressionMammalianMutationSerine 373 mutated to AlalninePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AICSDP-43: GJA1-mEGFP
Plasmid#109120PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1DepositorInsertGJA1 Homology Arms with linker-mEGFP (GJA1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-mCherry-GJA1-20k-N1
Plasmid#49861PurposeEncodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tagDepositorInsertGJA1-20k (GJA1 Human)
UseTagsmCherryExpressionMammalianMutationGJA1-20k corresponds to residues 213 to 382 to Co…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA
Plasmid#164471PurposeExpresses sgRNA targeting human GJA1 locusDepositorInsertGap junction alpha-1 (GJA1) (GJA1 Human)
UseTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
Cx43-msfGFP
Plasmid#69007PurposeExpresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertGja1 (Gja1 Rat)
UseTagsmonomerized superfolder GFPExpressionMammalianMutationPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
msfGFP-Cx43
Plasmid#69024PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSFFV-Cx43
Plasmid#69442Purposemammalian expression of rat Cx43DepositorInsertCx43 (Gja1 Rat)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-siResist
Plasmid#49852PurposeEncodes human connexin 43 with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cx43-sfGFP
Plasmid#70053PurposeExpresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsnon-monomerized superfolder GFP (V207)ExpressionMutationfused in frame to non-monomerized superfolder GFPPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx43
Plasmid#69020PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsEBFP2ExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3.2-Cx43-HA
Plasmid#49851PurposeEncodes human connexin 43 with a C-terminal HA epitope fusion tagDepositorInsertConnexin 43 (GJA1 Human)
UseTagsHAExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100L-siResist
Plasmid#49854PurposeEncodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100 mutated to Leucine. Contains severa…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP-Cx43ML-N1
Plasmid#49860PurposeEncodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tagDepositorInsertConnexin 43 (GJA1 Human)
UseTagsEGFPExpressionMammalianMutationAll internal Methionines mutated to Leucine. No s…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cx43K258stop-msfGFP
Plasmid#69023PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationdelete codons 258-381 and fuse in frame to monome…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLPCX-Cx43-IRES-GFP
Plasmid#65433PurposeMammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral productionDepositorInsertCx43 (GJA1 Human)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBS-Cx43-FS
Plasmid#65440PurposeCloning, T7 RNA expressionDepositorInsertCx43 (GJA1 Human)
UseT7 in vitro rna productionTagsExpressionBacterialMutationFrameshift (FS) mutation 35delAPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-NoStart
Plasmid#49853PurposeEncodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationStart codon mutated to from methionine to isoleuc…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 47 results