We narrowed to 128 results for: px459 plasmids

1. eIF3k sgRNA-2 PX459 plasmid

   Plasmid

   #192256

   Purpose

   expressing sgRNA-2 targeting eIF3k locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. eIF3k sgRNA-1 PX459 plasmid

   Plasmid

   #192255

   Purpose

   expressing sgRNA-1 targeting eIF3k locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. eIF3f sgRNA-1 PX459 plasmid

   Plasmid

   #192253

   Purpose

   expressing sgRNA-1 targeting eIF3f locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. eIF3f sgRNA-2 PX459 plasmid

   Plasmid

   #192254

   Purpose

   expressing sgRNA-2 targeting eIF3f locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. eIF3b sgRNA-2 PX459 plasmid

   Plasmid

   #192250

   Purpose

   expressing sgRNA-2 targeting eIF3b locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eIF3b sgRNA-1 PX459 plasmid

   Plasmid

   #192249

   Purpose

   expressing sgRNA-1 targeting eIF3b locus closed to stop coden

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. px459 sgFIP200

    Plasmid

    #175024

    Purpose

    CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    RB1CC1 (RB1CC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px459 EQR

    Plasmid

    #101732

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px459 sgAtg5

    Plasmid

    #175023

    Purpose

    CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

    Depositor

    David Tumbarello

    Article

    Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

    Insert

    ATG5 (ATG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX459-sgPLXNB2

    Plasmid

    #86151

    Purpose

    Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

14. pX459_GINS4_gRNA

    Plasmid

    #232734

    Purpose

    Generates GINS4 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    GINS4 (GINS4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX459_MCM2_sgRNA

    Plasmid

    #232730

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459_CDC45_sgRNA

    Plasmid

    #232732

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Martijn Verdoes, Ferenc Scheeren

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX459-puro-hGSDMD

    Plasmid

    #185377

    Purpose

    For mammalian expression of guide RNA: CGCGCCAGACGCGCCACCCT that targets human GSDMD (Gasdermin D)

    Depositor

    Ayaz Najafov

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX459-puro-hPKAalpha

    Plasmid

    #185379

    Purpose

    For mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alpha

    Depositor

    Ayaz Najafov

    Insert

    PRKACA (PRKACA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX459-puro-hMYC

    Plasmid

    #185376

    Purpose

    For mammalian expression of guide RNA: TGCTGCCAAGAGGGTCAAGT that targets human MYC

    Depositor

    Ayaz Najafov

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge