159,428 results

1. pLKO-PTEN-shRNA-1320

   Plasmid

   #25638

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PTEN-shRNA-1320 (PTEN Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. M13-kmR

   Plasmid

   #206861

   Purpose

   M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

   Depositor

   Irene Chen

   Article

   Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

   Insert

   Kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Use

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY1314_RADARS_Luciferase

   Plasmid

   #193191

   Purpose

   Express Gaussia Luciferase conditional upon RNA transcript

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   RADARS Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. C1(1-29)-TurboID-V5_pCDNA3

   Plasmid

   #107173

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-smURFP-RBS-HO-1

   Plasmid

   #80341

   Purpose

   Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   HO-1
   

   Use

   Tags

   6 Histidine Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b - PA

   Plasmid

   #84863

   Purpose

   bacterial expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Tags

   pelB signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUltra-U6-gRNAs1-5

   Plasmid

   #178569

   Purpose

   Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Insert

   5 gRNA-tRNA array with U6 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH2B_mCherry_IRES_puro2

    Plasmid

    #21045

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B (H2BC16P Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. EB3-tdTomato

    Plasmid

    #50708

    Purpose

    expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Article

    Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-miniTurbo

    Plasmid

    #124647

    Purpose

    Expresses Flag-miniTurbo fusion for proximity labeling

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1.tR4-MbPyl

    Plasmid

    #50832

    Purpose

    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

    Depositor

    Peter Schultz

    Article

    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

    Inserts

    two-copy Pyl tRNA cassette
    MbPyl
    two copy Pyl tRNA cassette
    

    Use

    Baculoviral

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CMV and U6 and H1

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide-puro-NLS-mCherry

    Plasmid

    #185474

    Purpose

    Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tg(mpeg1:LifeAct-mRuby)

    Plasmid

    #166977

    Purpose

    labels F-actin in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

    Inserts

    mpeg1 (mpeg1.1 Zebrafish)
    LifeAct (actb1 Frog)
    

    Use

    Tags

    Lifeact-mRuby and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

    Plasmid

    #225462

    Purpose

    Express mEGFP-fusion protein of FMR1 isoform 1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 isoform 1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

    Plasmid

    #154850

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 GFP LIC cloning vector (6D)

    Plasmid

    #30127

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFB2A

    Plasmid

    #195283

    Purpose

    Bicistronic DTR and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Hygro (HBEGF Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Farnselation tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. Epac-S-H187

    Plasmid

    #170348

    Purpose

    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK172-6xHis-0N4R-P301S-tau-HiBiT

    Plasmid

    #187835

    Purpose

    Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    6XHis-0N4R-P301S-tau-HiBiT
    

    Use

    Tags

    6xHistidine and HiBiT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTD103luxI_sfGFP

    Plasmid

    #48885

    Purpose

    Positive feedback plasmid from oscillator

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI, sfGFP, and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HA-Foxo1ADA (pCMV5)

    Plasmid

    #12143

    Depositor

    Domenico Accili

    Article

    Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

    Insert

    Foxo1 ADA (Foxo1 Mouse)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Phosphorylation-deficient, also referred to as co…

    Promoter

    CMV

    Available since

    Aug. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Use

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lenti-sgRNA blast

    Plasmid

    #104993

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-U6-sgRNA(CTRL)-CMV-eGFP

    Plasmid

    #194017

    Purpose

    Expresses a gRNA that targets the LacZ gene (serves as control) and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(CTRL)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-TBG-ER-TurboID

    Plasmid

    #149415

    Purpose

    Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    ER-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    TBG

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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