27,467 results
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Plasmid#138568PurposeUniversal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP2A-clover-BlastR
UseCRISPRAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR_L1-YFP-Turbo-L2
Plasmid#127350Purposegateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboID (BirA mutant)
UseGateway CloningTagsGS linker, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD2-EGFP
Plasmid#131207Purposemammalian expression, tet RDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNOD2 (NOD2 Human)
TagsEGFPExpressionMammalianPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARHGEF11(DHPH)-CRY2-mCherry
Plasmid#89481PurposeAlso known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsmCherryExpressionMammalianPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF6a-CD28-PafA
Plasmid#113400PurposeExpresses CD28-PafA in mammalian cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD28-PafA
TagsmycExpressionMammalianPromoterEF-1αAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Opto-CRAC version 1
Plasmid#101245PurposeGenetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signalingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLOV2 and STIM1 cytosolic fragment
TagsmCherryExpressionBacterial and MammalianPromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 1kb prom. CD274
Plasmid#107002PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0042-PguCas13b crRNA backbone
Plasmid#103853PurposeFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertU6-PguCas13b DR-BbsI-BbsI-polyT
UseCRISPRExpressionMammalianAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcrDNA3.1-PRKACA
Plasmid#100890PurposeMammalian expression of PRKACADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertProtein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
ExpressionMammalianMutationIsoform 1 wild typePromoterCMVAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_hTRIM32
Plasmid#69541PurposeExpresses human TRIM32 fused to c-terminal EGFPDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttripartite motif containing 32 (TRIM32 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-NSP2SA11
Plasmid#89169PurposeExpress Rotavirus SA11 NSP2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRotavirus SA11 NSP2
ExpressionMammalianPromoterT7 promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-4:TUBA1B-mEGFP
Plasmid#87421PurposeHomology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pKanCMV-mRuby3-18aa-Tubulin
Plasmid#74256PurposeMammalian expression of N-terminal fusion of mRuby3 to TubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTubulin
TagsmRuby3ExpressionMammalianPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CRY2-5ptaseOCRL
Plasmid#66836PurposeExpression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5ptaseOCRL (OCRL Human)
TagsmCherry-CRY2ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GABA(A) receptor subunit a2SE
Plasmid#49169PurposepHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGABA(A) receptor subunit alpha-2 (GABRA2 Human)
TagsHA, bovine alpha 1 signal sequence, and pHGFP (pH…ExpressionMammalianMutationT343APromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-mouse PKA-Calpha-mEGFP
Plasmid#45521DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKA catalytic subunit alpha tagged by mEGFP
ExpressionMammalianAvailable sinceJune 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEYFP-NR1a
Plasmid#17928DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertN-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
TagsEYFPExpressionMammalianAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3xFLAG-SENP7
Plasmid#42886DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSENP7 (SENP7 Human)
Tags3xFLAGExpressionMammalianMutationT102A, R969C, L996SAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ai34
Plasmid#34881DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCAG-Floxed Synaptophysin-tdTomato
UseMouse TargetingTagstdTomatoPromoterCAGAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAXEF-Rap1-E63(GTP)
Plasmid#32698DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRAP1 (RAP1A Human)
ExpressionMammalianMutationE63: Consitutively GTP-boundPromoterEF promoterAvailable sinceDec. 5, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by