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27,466 results

Showing: 3561 - 3580 of 27466 results

  2. pcDNA3-ExRai-CKAR

    Plasmid
    #118409
    Purpose
    cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-CKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    118409-plasmid-map-sequence-id-243085

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  7. pX-evoCas9

    Plasmid
    #107550
    Purpose
    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells
    Depositor
    Anna Cereseto
    Publication
    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    M495V/Y515N/K526E/R661Q
    Promoter
    CBh
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107550-plasmid-map-sequence-id-206015

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  9. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid
    #99315
    Purpose
    Negative control for luciferase validation vectors
    Depositor
    Alexander Stark
    Publication
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    neutral region; chr3: 55198740-55200041
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99315-plasmid-map-sequence-id-193174

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  13. pUBC_V5_MS2-mCherry

    Plasmid
    #68420
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2
    Use
    Synthetic Biology
    Tags
    V5 and mCherry
    Expression
    Mammalian
    Mutation
    MS2: V75E/A81G
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68420-plasmid-map-sequence-id-511194

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  15. Str-KDEL_ManII-SBP-EGFP

    Plasmid
    #65252
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65252-plasmid-map-sequence-id-120582

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  17. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64710-plasmid-map-sequence-id-108923

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  19. PX852

    Plasmid
    #62884
    Purpose
    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62884-plasmid-map-sequence-id-105906

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  20. PX851

    Plasmid
    #62883
    Purpose
    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62883-plasmid-map-sequence-id-105905

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Showing: 3561 - 3580 of 27466 results