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27,466 results

Showing: 4021 - 4040 of 27466 results

  6. pCMV-PE7-P2A-BSD

    Plasmid
    #224122
    Purpose
    Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-P2A-BSD
    Tags
    P2A-BSD, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    224122-plasmid-map-sequence-id-442586

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  8. pcDNA3-cytoEKAR4

    Plasmid
    #174438
    Purpose
    Cytosol-targeted EKAR4.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cytoEKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174438-plasmid-map-sequence-id-367724

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  9. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Publication
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    168122-plasmid-map-sequence-id-332862

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  14. pCMV-dCas13-inactive M3nls

    Plasmid
    #157854
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    157854-plasmid-map-sequence-id-307627

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  19. pEGFP-N1 hMOG-alpha1-EGFP

    Plasmid
    #126463
    Purpose
    Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells
    Depositor
    Markus Reindl
    Publication
    Mader et al J Neuroinflammation. 2011 Dec 28;8:184.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Myelin oligodendrocyte glycoprotein (MOG Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126463-plasmid-map-sequence-id-244968

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  20. KRAB-dCas9

    Plasmid
    #112195
    Purpose
    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAB (ZNF10 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-KRAB-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112195-plasmid-map-sequence-id-213538

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Showing: 4021 - 4040 of 27466 results