27,466 results
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Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTornado-CVB3-split-nLuc
UseLuciferaseExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Promoter Vector
Plasmid#212939PurposeControl vector with constitutive firefly luciferase expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertluc+
UseLuciferaseMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
NbV5-eGFP
Plasmid#201473PurposeIndirect microscopy/imaging of V5-tagged proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNbV5-eGFP
TagseGFPExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA C12 ecDHFR DD YFP HA
Plasmid#192815PurposeExpresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertE. coli dihydrofolate reductase
Tagsenhanced yellow fluorescent protein and hemagglut…ExpressionMammalianMutationW74R/T113S/E120D/Q146LPromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC3.1-caggs-Crimson-CAAX
Plasmid#190041PurposeRed Fluorescent Protein for Long-Term Labeling of Fine Structures in NeuronsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCrimson
TagsCAAXExpressionMammalianPromotercaggsAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNme2ABE8e
TagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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LifeAct-HaloTag
Plasmid#176105PurposeF-actin markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeAct
ExpressionMammalianAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
Plasmid#129631PurposeVisualization of Akt and ERK activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
ExpressionMammalianPromoterCMVAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA
Plasmid#171383PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized minidCas13X.1-REPAIRv2
TagsHAExpressionMammalianPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRN120
Plasmid#160696PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCmYLCV::Luc+::AtHSP
Nos::WUS2::PinII
AtUbi10::STM::35S
UseLuciferase; Gemini viral replicon, plant developm…PromoterAtUbi10, CmYLCV, and NosAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαq*-BERKY3
Plasmid#158217PurposeBRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
ExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-OMP25
Plasmid#141150PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOMP25 (Synj2bp Rat)
TagsAcGFPExpressionMammalianMutationOMP25 c terminus from addgene plasmid #69598PromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-GGamma9-GFP2
Plasmid#140991PurposeEncodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGGamma9-GFP2 (GNGT2 Human)
TagsGFP2 and GSAG linkerExpressionMammalianPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hCMV-IE1:Renilla
Plasmid#118066PurposeTranscriptional unit for constitutive expression of Renilla in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV:GreenRenilla:bGHF
UseLuciferase and Synthetic Biology; Assembly of gb2…ExpressionMammalianPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-UFM1
Plasmid#134639Purposeexpression of HA-tagged human UFM1 wildtypeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUFM1 (UFM1 Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
UbC-(mNeonGreen)4-tDeg
Plasmid#129404Purpose(mNeonGreen)4-tDeg fluorogenic proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert(mNeonGreen)4-tDeg
ExpressionMammalianPromoterUbCAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
negMaLionR
Plasmid#113909Purposenegative control for Red ATP sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnegMaLionR
ExpressionMammalianAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only