27,006 results
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Plasmid#168266PurposeA single-component optogenetic tool for photoswitchable pyroptosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmGSDMD(1-276) (Gsdmd Mouse)
TagscpLOV2, mCherryExpressionMammalianMutationR138A, K146A, R152A, R154APromoterCMVAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGFP-P2A-H2AmCherryPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-Tox-IRES-eGFP
Plasmid#165427PurposeExpresses TOX in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOX (Tox Mouse)
Tags3xflag 5' of ToxExpressionMammalianPromoter5' LTRAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-JIP3
Plasmid#164624PurposeExpresses JIP3 with a GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJIP3 (MAPK8IP3 Human)
Tagscycle 3 GFPExpressionMammalianPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-TNFa-RUSH
Plasmid#166901PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-HaloDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHaloTag
ExpressionMammalianAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEGFP-N1-YAPS127A
Plasmid#166458PurposeExpresses fusion of mEGFP and YAPS127ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmEGFP, YAPS127A (YAP1 Human)
ExpressionMammalianAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ORF3a-EGFP
Plasmid#165121Purposemammalian expression and localizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertORF3a (ORF3a codon optimized SARS-COV-2)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lys-eMagA-EGFP
Plasmid#162246PurposeBlue-light dependent heterodimerization systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp18-eMagA-EGFP
ExpressionMammalianAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Neo)
Plasmid#140645PurposeCTCF tagging with mAID-CloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTCF (CTCF Human)
UseCRISPRExpressionMammalianAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-gRNA
Plasmid#158973PurposeA human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA3
ExpressionMammalianPromoterU6Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-His:dCas9:nls:10P3
Plasmid#135982PurposeExpresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9 fused to 10 copies of the P3 coiled-coil
UseCRISPRTagsHisExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA.3.1.ADRB2-NP
Plasmid#134376PurposeNanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADRB2-NP (ADRB2 Human)
TagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianPromoterT7Available sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
ExpressionMammalianAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1.Gα12-LgB115
Plasmid#134363PurposeNanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGα12-LgB115 (GNA12 Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PI4KB
Plasmid#139310PurposeRecruitable PI4KBDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)
ExpressionMammalianAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-LSD1-Hygro
Plasmid#104406Purposetransient expression of dCas9-LSD1 fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSD1 (KDM1A Human)
UseCRISPRExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EMTB-mScarletI
Plasmid#137801PurposeVisualization of microtubulesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEnsconsin microtubule binding domain
ExpressionMammalianPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)
Plasmid#139994PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP
Plasmid#138334PurposeExpresses APOB-Linker-eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5'UTR-ApoB48-Linker-eGFP (APOB Human)
TagseGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by