27,006 results
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Plasmid#136926PurposeExpresses Cas9-NRCH in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9-NRCH
UseCRISPRExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-Kaede
Plasmid#132972Purposefull-length of photoconvertible kaede with stop codon in pmE Gateway vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKaede
UseGateway vectorAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TGN38 EGFP
Plasmid#128148PurposeExpression of TGN38 amino acids 304-357DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTGN38 (Tgoln2 Rat)
TagsmEGFPExpressionMammalianMutationContains amino acids 304-357Available sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Beta-Globin-WT
Plasmid#130700PurposeExpresses Beta-Globin WT NMD reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBeta Globin (HBB Human)
ExpressionMammalianPromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-TRIM28deltaPB
Plasmid#124959PurposeMammalian expression of Human TRIM28 with deleted PHD and BROMO domainDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthTRIM28 (TRIM28 Human)
TagsHAExpressionMammalianMutationDeletion of PHD and Bromo DomainAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBT120b
Plasmid#122604PurposeBE PACE selection, express gIII, luxAB and degron-GTCCA guide RNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgIII, luxAB, guide RNA
UseSynthetic BiologyAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT376
Plasmid#125617PurposeExpresses BE4max-NG in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE4max-NG
ExpressionMammalianAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Influenza-FPV-HA-mEGFP
Plasmid#127810PurposeExpression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInfluenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
ExpressionMammalianAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMF1762
Plasmid#125583PurposeExpresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmt-roGFP2
ExpressionMammalianPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
STING-V2
Plasmid#124264PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTMEM173 (STING1 Human)
TagsVenus2ExpressionMammalianAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ArchT-BFP2-TSERex
Plasmid#123312PurposeExpresses the proton pump rhodopsin in mammalian cells under CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArchT-BFP2
ExpressionMammalianPromoterCAGAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
cycLuc_TEVS
Plasmid#119207PurposeCircularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcyclic luciferase with TEVs cleavage site
UseLuciferaseTags6xHis and AU1 tagExpressionMammalianPromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-T2A-HygR
Plasmid#118153PurposeSpCas9 expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9-T2A-HygR
UseCRISPRTags3XFLAGExpressionMammalianPromoterCAG and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PYL1_nTEVp
Plasmid#119213PurposeExpresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsplit nTEVp in sufion with PYL1
TagsAU1 tagExpressionMammalianPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Plasmid#121185PurposeOsTIR1 introductionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTet-OsTIR1-loxP-PURO-loxP
UseCRISPRMutationOsTIR1 codon optimized for human cellsAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/Envelope
Plasmid#120821PurposeEctopic expression of ZIKV proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEnvelope ZIKV
TagsV5ExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR
Plasmid#118468Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsapphireCKAR
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q79
Plasmid#111729PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ79 (polyQ repeat)PromoterCMV and P10Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by