Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 9261 - 9280 of 30881 results

  3. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

    Enlarge

  4. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

    Enlarge

  5. pFB2F

    Plasmid
    #195289
    Purpose
    Bicistronic HSV-TK and mScarlet resistance for positive/negative selection
    Depositor
    Scott Lowe
    Publication
    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HSV-TK 2A mScarlet
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    195289-plasmid-map-sequence-id-382900

    Enlarge

  6. pCMV-APOBEC1-YTHD422N

    Plasmid
    #194432
    Purpose
    APOBEC1 fused to YTHD422N
    Depositor
    Kate Meyer
    Publication
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    APOBEC1-YTHD422N (APOBEC1 Human)
    Use
    CRISPR
    Tags
    APOBEC1-YTHD422N-HA
    Expression
    Mammalian
    Mutation
    D422N mutation in the YTH domain to enhance m6A b…
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194432-plasmid-map-sequence-id-382432

    Enlarge

  9. pDL025_HA-rb_IgG_HC_15F11

    Plasmid
    #193622
    Purpose
    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.
    Depositor
    Jennifer DeLuca
    Publication
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Anti_HA_15F11 rabbit heavy chain
    Tags
    signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193622-plasmid-map-sequence-id-446783

    Enlarge

  12. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

    Enlarge

  15. pCAGGS SARS-CoV-2 BA.2.75 Spike

    Plasmid
    #190012
    Purpose
    Expresses codon-optimized full length SARS-CoV-2 BA.2.75 Spike
    Depositor
    Marceline Côté
    Publication
    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 BA.2.75 Spike (S )
    Expression
    Mammalian
    Mutation
    Contains the BA.2 mutations (T19I, LPPA24S, G142D…
    Promoter
    chicken β-actin promoter, CMV enhancer
    Available since
    Sept. 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    190012-plasmid-map-sequence-id-371147

    Enlarge

  19. CAG-rtTA

    Plasmid
    #185562
    Purpose
    Ubiquitous expression of reverse tetracycline transactivator for TetON system
    Depositor
    Connie Cepko
    Publication
    Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rtTA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    185562-plasmid-map-sequence-id-365051

    Enlarge

  20. pcDNA3-ICUE4

    Plasmid
    #181846
    Purpose
    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ICUE4 (RAPGEF3 Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Glutamine 122 in Epac1 domain mutated to glutamat…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181846-plasmid-map-sequence-id-364236

    Enlarge

Showing: 9261 - 9280 of 30881 results