Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 9261 - 9280 of 27462 results

  1. pES.42.1c

    Plasmid
    #69576
    Purpose
    TCR a chain cDNA in pES4 (transgenic construct) for creating OT-1 mice
    Depositor
    Francis R. Carbone
    Publication
    Hogquist et al Cell. 1994 Jan 14;76(1):17-27.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TCR alpha clone 149.42 (Tcra Mouse)
    Expression
    Mammalian
    Promoter
    H-2Kb
    Available since
    Sept. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    68417-plasmid-map-sequence-id-120195

    Enlarge

  3. MAS1-Tango

    Plasmid
    #66425
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAS1 (MAS1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. GPR39-Tango

    Plasmid
    #66357
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR39 (GPR39 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66357-plasmid-map-sequence-id-456562

    Enlarge

  5. GPR123-Tango

    Plasmid
    #66311
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR123 (ADGRA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66311-plasmid-map-sequence-id-456481

    Enlarge

  6. GPR149-Tango

    Plasmid
    #66324
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR149 (GPR149 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. GAL1-Tango

    Plasmid
    #66288
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL1 (GALR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66288-plasmid-map-sequence-id-247685

    Enlarge

  8. GNRHR-Tango

    Plasmid
    #66297
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNRHR (GNRHR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. CRHR2-Tango

    Plasmid
    #66257
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRHR2 (CRHR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. Oct4-Cr1

    Plasmid
    #66989
    Purpose
    Expresses gRNA that targets stop codon for OCT4
    Depositor
    Danwei Huangfu
    Publication
    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA for Crispr targeting the OCT4 stop codon
    Expression
    Mammalian
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. RhoC FLARE.sc mCer, mVenus - wt

    Plasmid
    #65071
    Purpose
    RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC
    Depositor
    Klaus Hahn
    Publication
    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RBD, mCerulean, mVenus, RhoC
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    T153M in mVenus
    Promoter
    CMV, p10
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  17. EGFP-BICD1

    Plasmid
    #49487
    Purpose
    expresses EGFP-BICD1 in mammalian cells
    Depositor
    Marci Scidmore
    Publication
    Moorhead et al Infect Immun. 2007 Feb;75(2):781-91. Epu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BICD1 (BICD1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    ** see notes
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. Str-STIM1-NN_ST-SBP-mCherry

    Plasmid
    #65263
    Purpose
    synchronize trafficking of ST from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    Tags
    Streptavidin Binding Protein (SBP) and mCherry
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 43 of ST
    Promoter
    CMV
    Available since
    June 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65263-plasmid-map-sequence-id-110471

    Enlarge

Showing: 9261 - 9280 of 27462 results