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27,462 results

Showing: 11121 - 11140 of 27462 results

  1. PGK 4xMito-mEmerald

    Plasmid
    #200430
    Purpose
    Mammalian expression of fluorescently-tagged marker for mitochondria
    Depositor
    Erika Holzbaur
    Publication
    Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    4x mito-targeting sequence
    Promoter
    PGK
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    200430-plasmid-map-sequence-id-393638

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  2. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid
    #199255
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1) nuclease
    Depositor
    Manuel Goncalves
    Publication
    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1) nuclease
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199255-plasmid-map-sequence-id-391368

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  3. mBFP-APPP1-mGFP

    Plasmid
    #196695
    Purpose
    As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    mEGFP and mtagBFP2
    Expression
    Mammalian
    Mutation
    Lys to Val substitution at position 612
    Promoter
    CMV
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196695-plasmid-map-sequence-id-386451

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  4. mCherry-APPP1-mGFP

    Plasmid
    #196705
    Purpose
    As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    mCherry and mEGFP
    Expression
    Mammalian
    Mutation
    Lys to Val substitution at position 612
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196705-plasmid-map-sequence-id-391079

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  5. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Publication
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Promoter
    sp6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

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  6. Membrane EGFP

    Plasmid
    #198056
    Purpose
    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LCK membrane EGFP (LCK Human, A. victoria, Synthetic)
    Use
    Sea urchin, mammal, zebrafish
    Tags
    EGFP
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    198056-plasmid-map-sequence-id-389155

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  9. opto-Cdc42

    Plasmid
    #174510
    Purpose
    Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    opto-Cdc42-mCherry
    Tags
    3xFLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    174510-plasmid-map-sequence-id-357203

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  10. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Publication
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    194328-plasmid-map-sequence-id-383152

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  11. pGL4_10xUAS-MLPmin-luc2

    Plasmid
    #194383
    Purpose
    Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter
    Depositor
    Michael Wehr
    Publication
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    10xUAS-luc2
    Expression
    Mammalian
    Promoter
    MLP minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    194383-plasmid-map-sequence-id-381605

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  17. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

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  19. His-FLAG-Tev_ACTB (beta-actin)

    Plasmid
    #188453
    Purpose
    Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.
    Depositor
    Roberto Dominguez
    Publication
    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB (ACTB Human)
    Tags
    6xHis-FLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188453-plasmid-map-sequence-id-373094

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  20. EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191156
    Purpose
    Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR
    Depositor
    Xiaojing Gao
    Publication
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:GAPDH.s1:EGFP-3xMS2
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191156-plasmid-map-sequence-id-375490

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Showing: 11121 - 11140 of 27462 results