Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 11301 - 11320 of 27462 results

  1. dSp-VP64-p65(100-261)-RTA(125-190)

    Plasmid
    #99673
    Purpose
    Expresses dSp Cas9 fused to combination of truncated activation domains
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Tags
    VP64-p65(101-261)-RTA(125-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    99673-plasmid-map-sequence-id-192082

    Enlarge

  3. RAB11A-mCherry HDRT Source (pTR 144)

    Plasmid
    #112013
    Purpose
    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry
    Depositor
    Alexander Marson
    Publication
    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB11A-mCherry HDRT (RAB11A Human)
    Use
    CRISPR and Synthetic Biology
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112013-plasmid-map-sequence-id-215522

    Enlarge

  4. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Publication
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Available since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113643-plasmid-map-sequence-id-217116

    Enlarge

  5. p3s-ABE7.10

    Plasmid
    #113128
    Purpose
    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized
    Depositor
    Jin-Soo Kim
    Publication
    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE 7.10
    Tags
    HA epitope and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    113128-plasmid-map-sequence-id-216920

    Enlarge

  6. EGFP-PH

    Plasmid
    #96948
    Purpose
    EGFP-PLC pleckstrin homology domain for CALI validation
    Depositor
    Marcel Bruchez
    Publication
    He et al Nat Methods. 2016 Mar;13(3):263-268. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Pleckstrin homology domain
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    96948-plasmid-map-sequence-id-202205

    Enlarge

  7. VinculinTS-I997A

    Plasmid
    #111828
    Purpose
    Vinculin tension sensor with point mutation affecting ability to bind actin
    Depositor
    Brenton Hoffman
    Publication
    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VinculinTS (I997A)
    Expression
    Mammalian
    Mutation
    changed isoleucine 997 to alanine
    Promoter
    CMV
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    111828-plasmid-map-sequence-id-222593

    Enlarge

  14. pEGFP-N1_MeCP2(deltaNIC)

    Plasmid
    #110191
    Purpose
    Expression of mouse MeCP2 (deltaNIC) in mammalian cells
    Depositor
    Adrian Bird
    Publication
    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    delta N and C termini. Intervening region replace…
    Promoter
    CMV
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110191-plasmid-map-sequence-id-210799

    Enlarge

  15. RINS1(mut)

    Plasmid
    #107291
    Purpose
    Plasmid for the expression of the inactive mutant of RINS1 sensor
    Depositor
    Dmytro Yushchenko
    Publication
    Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RINS1(mut)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    two Arg/Ser mutations at the beginning and in the…
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107291-plasmid-map-sequence-id-209471

    Enlarge

  16. pUBC_hsPUM1-iRFP670

    Plasmid
    #107609
    Purpose
    Transient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoter
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsPUM1-iRFP670
    Use
    Synthetic Biology
    Tags
    iRFP670
    Expression
    Mammalian
    Promoter
    human UBC
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107609-plasmid-map-sequence-id-207515

    Enlarge

  17. pCAGGS-6017nes

    Plasmid
    #108655
    Purpose
    A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
    Depositor
    Michiyuki Matsuda
    Publication
    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hyBRET-PKA-EV
    Tags
    XFP
    Expression
    Mammalian
    Promoter
    pCAGGS
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108655-plasmid-map-sequence-id-208324

    Enlarge

  19. pCEP4-HA-CCR5

    Plasmid
    #98950
    Purpose
    Mammalian expression plasmid for N-terminal HA-tagged human CCR5
    Depositor
    Erik Procko
    Publication
    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CCR5 (CCR5 Human)
    Tags
    HA (YPYDVPDYA) epitope tag and Signal/leader sequ…
    Expression
    Mammalian
    Mutation
    Full-length, wildtype
    Promoter
    CMV
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98950-plasmid-map-sequence-id-193408

    Enlarge

  20. PLK1-cjun-FRET-PACT biosensor

    Plasmid
    #106907
    Purpose
    centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)
    Depositor
    Heidi Hehnly
    Publication
    Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)
    Tags
    PACT domain
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106907-plasmid-map-sequence-id-208145

    Enlarge

Showing: 11301 - 11320 of 27462 results