Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 11381 - 11400 of 27462 results

  3. pCAG-Venus-MDGA1

    Plasmid
    #82543
    Purpose
    Overexpression vector for Venus-MDGA1 in neurons
    Depositor
    Alice Ting
    Publication
    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MDGA1 (MDGA1 Human)
    Tags
    Venus fluorescent protein
    Expression
    Mammalian
    Promoter
    chicken beta actin
    Available since
    Sept. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    82543-plasmid-map-sequence-id-156675

    Enlarge

  8. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Publication
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80439-plasmid-map-sequence-id-151455

    Enlarge

  9. pCAG_EGFP-RandE3

    Plasmid
    #79870
    Purpose
    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.
    Depositor
    Don Arnold
    Publication
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-RandE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Promoter
    Beta-actin
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    79870-plasmid-map-sequence-id-149706

    Enlarge

  10. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Matthew Alexander, Louis Kunkel
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SRF (SRF Human)
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pcDNA3_erGAP2

    Plasmid
    #78120
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Teresa Alonso, Javier García-Sancho
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    erGAP2
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    D24N/D119A in aequorin moiety
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    78120-plasmid-map-sequence-id-153381

    Enlarge

  15. TetO-dCas9-mD3A

    Plasmid
    #78255
    Purpose
    Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter
    Depositor
    Grant Challen
    Publication
    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3A CD aa 598-912 (DNMT3A Human)
    Use
    CRISPR
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Mutation
    E756A
    Promoter
    CMV
    Available since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    78255-plasmid-map-sequence-id-150192

    Enlarge

  16. pRK-flag-USP19 1-1290

    Plasmid
    #78579
    Purpose
    Expression flag-USP19 1-1290 in mammalian cells
    Depositor
    Yihong Ye
    Publication
    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    flag-USP19 1-1290 (USP19 Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    mutate 1291 residue to stop condon
    Promoter
    CMV
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  19. pcDNA3.1-GNPTAB

    Plasmid
    #78107
    Purpose
    Mammalian expression of human GNPTAB, the alpha/beta subunit precursor miniconstruct that allows its efficient expression, spans the membrane twice, and lacks amino acids 431 to 819.
    Depositor
    Thomas Braulke
    Publication
    Marschner et al Science. 2011 Jul 1;333(6038):87-90. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNPTAB (GNPTAB Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 11381 - 11400 of 27462 results