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pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pKD-SepRS-EFSep

Plasmid

#34623

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Inserts

SepRS (MMP0688 Methanocaldococcuc maripaludis)
EF-Sep (tufB E. coli)

Expression

Bacterial

Mutation

His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

Promoter

Ptac and Ptac (from SepRS- operon like)

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pAB46 (CK2beta)

Plasmid

#27085

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2beta (CSNK2B Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGGD_RT_SRDX

Plasmid

#136976

Purpose

Goldengate/Greengate module EAR-repression domain

Depositor

Klaus Theres

Insert

SRDX

Expression

Bacterial

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pet21_TurboID_18A

Plasmid

#170922

Purpose

Express TurboID_18A in Bl21

Depositor

Huan Bao

Article

Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

Insert

TurboID-18a

Expression

Bacterial

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.Y

Plasmid

#201004

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

sYFP2

Expression

Bacterial

Promoter

J23100

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023

Plasmid

#90324

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAD51_Rad51

Plasmid

#110031

Purpose

Protein expression and purification of RAD51_Rad51

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAD51_Rad51 (RAD51 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI

Plasmid

#115268

Purpose

Expresses EiCsm6 from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

EiCsm6

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Dlx2

Plasmid

#15537

Depositor

John Rubenstein

Article

Porteus et al Neuron. 1991 Aug . 7(2):221-9.

Insert

Dlx2 (Dlx2 Mouse)

Expression

Bacterial

Mutation

Partial cDNA

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
BLA_CAT_K136_GPG_LOV2

Plasmid

#231575

Purpose

This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

CAT_ carrying an AsLOV2 insertion behind K136

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-SYFP2

Plasmid

#189762

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_YES1_SH3

Plasmid

#178492

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

YES1_SH3 (YES1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pmr101A-cPr-mNeonGreen (ErmR)

Plasmid

#177208

Purpose

Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

Depositor

Alexey Merz

Insert

mNeonGreen

Expression

Bacterial

Mutation

Yeast codon optimized

Promoter

Synthetic constitutive promoter

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pThermoCas9_ctrl

Plasmid

#100981

Purpose

Expresses ThermoCas9 and its sgRNA module

Depositor

John van der Oost

Article

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Tags

None

Expression

Bacterial

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Dlx1

Plasmid

#15536

Depositor

John Rubenstein

Article

Bulfone et al J Neurosci. 1993 Jul . 13(7):3155-72.

Insert

Dlx1 (Dlx1 Mouse)

Expression

Bacterial

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
pET28a_Pro-IL-33

Plasmid

#214342

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL33 (IL33 Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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