30,738 results

1. pET24TEV-tSHIP1

   Plasmid

   #183770

   Purpose

   Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

   Depositor

   William Kerr

   Article

   Sudan et al Front Immunol. 2022 May 4;13:830961. doi

   Insert

   SHIP1 (INPP5D Human)
   

   Tags

   6HIS and Tobacco Etch Virus (TEV) Cleavage site

   Expression

   Bacterial

   Mutation

   includes aa1 to aa883 only

   Promoter

   T7 lac

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEANHISECArdB

   Plasmid

   #96967

   Purpose

   Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   E. coli ArdB
   

   Tags

   N-ter TEV cleavable 6His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVRa14_3200

   Plasmid

   #49666

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF14_3200
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDB1 (CK2alpha)

   Plasmid

   #27083

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha (CSNK2A1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRS-BG42-GFP

   Plasmid

   #220202

   Purpose

   mini-Tn7 GFP expression vector - pBG42 promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   monomeric superfolder green fluorescent protein
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   monomeric superfolder green fluorescent protein

   Promoter

   pBG42

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB180

   Plasmid

   #80677

   Purpose

   for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

   Depositor

   David Baker

   Article

   Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

   Type

   Empty backbone

   Tags

   OsmY-10His-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p406TDH3

   Plasmid

   #15977

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   TDH3 promoter is between SacI and XbaI restrictio…

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINJ

   Plasmid

   #26045

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   HisGST3C-lacZ
   

   Tags

   MAHHHHHH_GST_LEVLFQGT

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

   Plasmid

   #29722

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mC…

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-3c-rhVTN_FL

    Plasmid

    #30223

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin without deletions (VTN Human)
    

    Expression

    Bacterial

    Mutation

    No deletions

    Available Since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOxyR_rfp

    Plasmid

    #194155

    Purpose

    Red Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
    

    Expression

    Bacterial

    Promoter

    PoxyR, PoxyS

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_DYNLL1_Dynein-light

    Plasmid

    #110006

    Purpose

    Protein expression and purification of DYNLL1_Dynein-light

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DYNLL1_Dynein-light (DYNLL1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPP085

    Plasmid

    #158795

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET246-pUC57 12xMS2V6

    Plasmid

    #104390

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. ampkar

    Plasmid

    #35097

    Purpose

    A fluorescent reporter of AMPK activity and cellular energy stress

    Depositor

    Lewis Cantley

    Article

    Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

    Insert

    ampkar
    

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    na

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACλCI-β-flap (831-1057)

    Plasmid

    #53733

    Purpose

    Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    λCI-β-flap fusion
    

    Expression

    Bacterial

    Promoter

    lac UV5

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSPAsp-hp

    Plasmid

    #48122

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    predicted optimal signal peptide for Bacillus megaterium protein secretion
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBRα-β flap (831-1057)

    Plasmid

    #53734

    Purpose

    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    alpha NTD-Beta flap fusion
    

    Expression

    Bacterial

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAW014-2

    Plasmid

    #85612

    Purpose

    Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    ugtP-gRNA.P395T
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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