29,693 results

1. SPLICSFAST-ER-PO-P2A

   Plasmid

   #214894

   Purpose

   Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PO-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET32a-hEPR

   Plasmid

   #199233

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Proepiregulin preproprotein (EREG Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKB232

   Plasmid

   #170019

   Purpose

   Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   CRAMP
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_TXN_Thioredoxin

   Plasmid

   #109778

   Purpose

   Protein expression and purification of TXN_Thioredoxin

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   TXN_Thioredoxin (TXN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGPD2

   Plasmid

   #43972

   Depositor

   Hal Alper

   Article

   Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GPD

   Available since

   April 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTP1112

   Plasmid

   #104158

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

   Plasmid

   #73404

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   At4CL
   PhCHS
   CmCHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYTK095

   Plasmid

   #65202

   Purpose

   Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTlpA39-Wasabi

   Plasmid

   #86116

   Purpose

   mWasabi reporter gene gated by TlpA39

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA39
   mWasabi
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A, D135V, A217V, L236F

   Promoter

   pTlpA and pTlpA, LacI

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM8317

    Plasmid

    #213989

    Purpose

    PrpsM driven expression of sfGFP

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAWPSG-Ptet-nCas9-BE

    Plasmid

    #195739

    Purpose

    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet(Tetracycline inducible protein)

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pExp-sfGFP

    Plasmid

    #112573

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRKH3-slpGFP

    Plasmid

    #27168

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD_His B_QuasAr2

    Plasmid

    #64134

    Purpose

    Fluorescent reporter of membrane voltage with improved sensitivity

    Depositor

    Adam Cohen

    Article

    Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

    Insert

    QuasAr2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBSK

    Plasmid

    #67504

    Purpose

    Express the tRNALys scaffold

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid

    #60866

    Purpose

    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    CohIII
    CBM
    

    Use

    Tags

    6xHIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Cysteine 63 to Serine in CBM module

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNH-TrxT-G-L11

    Plasmid

    #127831

    Purpose

    Production of recombinant human ribosomal protein L11

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPL11 (RPL11 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST-2K

    Plasmid

    #44564

    Purpose

    for ectopic constitutive expression of two mycobacterial proteins in one vector

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. NylCA_pET28a

    Plasmid

    #215418

    Purpose

    Expression construct for NylCA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge