Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

31,436 results

Showing: 10821 - 10830 of 31436 results

  4. pRSETA_crE6G2

    Plasmid
    #172929
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain
    Depositor
    Arnold Boersma
    Article
    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.
    Insert
    CrowdingSensorE6G2
    Tags
    His
    Expression
    Bacterial
    Mutation
    Val317Gly, Met316Gly
    Promoter
    T7
    Available Since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172929-plasmid-map-sequence-id-339474

    Enlarge

  5. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

    Enlarge

  8. FGD5

    Plasmid
    #28109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FGD5:HPC09X-H08:C212499 (FGD5 Human)
    Tags
    His-tag with TEV site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 889-1304.
    Available Since
    March 21, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. J-HARS-4

    Plasmid
    #166529
    Purpose
    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166529-plasmid-map-sequence-id-331465

    Enlarge

Showing: 10821 - 10830 of 31436 results