33,434 results
-
Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
ExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneTagsFLAG and GSTExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pTP434
Plasmid#104167PurposeBacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
Tags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation3x Cysteines located at bps 493-495, 520-522, and…Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1
Plasmid#102630PurposeIn vitro expression of mRFP1 from the T7 promoterDepositorInsertmRFP1
ExpressionBacterialPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mTagBFP2
Plasmid#102638PurposeIn vitro expression of mTagBFP2 from the T7 promoterDepositorInsertmTagBFP2
ExpressionBacterialPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSET-dCas9
Plasmid#110183PurposeExpression of the dCas9 gene in StreptomycesDepositorInsertdCas9
UseCRISPR; IExpressionBacterialMutationD10A, H840APromoterermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_50
Plasmid#109248PurposeincW BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pRSETB
Plasmid#109484Purposebacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
TagsN-terminal histidine tag in vectorExpressionBacterialPromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR8k-GFPuv
Plasmid#106385PurposeL-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
ExpressionBacterialPromoteraraBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmPylRS(IPYE)
Plasmid#104073PurposeExpression plasmid encoding MmPylRS(IPYE) and pylTDepositorInsertPlpp MmPylRS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSW21
Plasmid#86836Purposebacterial expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
ExpressionBacterialPromoteraraBADAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ZIKV_Sensor_27B_LacZ
Plasmid#75006PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_27B_LacZ
TagsLacZExpressionBacterialPromoterT7Available sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHLUM (version 2)
Plasmid#64166Purposeharbours marker genes HIS3, LEU2, URA3 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCP30T-eCFP
Plasmid#78476PurposeBroad range bacterial fluorescence labeling (ECFP)DepositorInserteCFP
ExpressionBacterialAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His10 TEV LIC cloning vector (2B-T-10)
Plasmid#78173DepositorTypeEmpty backboneTags10xHis and TEV cleavage siteExpressionBacterialPromoterT7Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TOPO Cre-2A-GFP
Plasmid#68450PurposeTOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible geneDepositorInsertCre-2A-GFP
UseGmapExpressionBacterialPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig2
Plasmid#64842PurposeA lentiviral vector for the expression of mouse Olig2 under doxycyline controlDepositorAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRC319
Plasmid#61272PurposeAlso referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by