32,387 results

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HSA3_His

Plasmid

#202765

Purpose

human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA3

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19 Giles integrase

Plasmid

#170049

Purpose

pUC19 with Giles integrase

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Insert

GIles Integrase

Expression

Bacterial

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHex

Plasmid

#124670

Purpose

Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHex

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRK172-6xHis-0N4R-P301S-tau-HiBiT

Plasmid

#187835

Purpose

Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

6XHis-0N4R-P301S-tau-HiBiT

Tags

6xHistidine and HiBiT

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEMG-GRIS

Plasmid

#225134

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PGL4.11-COL1A1mSMAD

Plasmid

#230917

Purpose

Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)

Use

Luciferase

Tags

Luciferase-luc2p

Expression

Bacterial and Mammalian

Mutation

COL1A1 promoter with mutant potential SMAD bindin…

Promoter

COL1A1

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pES002 MBP-GSK3β_S9A-HA-His

Plasmid

#196184

Purpose

Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Insert

GSK3B (GSK3B Human)

Tags

HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBHM2641

Plasmid

#229525

Purpose

Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.

Depositor

Hiten Madhani

Insert

OsTIR1

Use

CRISPR

Tags

t2a-mNeonGreen-AID*

Expression

Bacterial and Yeast

Mutation

F74G

Promoter

TEF1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2644

Plasmid

#229528

Purpose

Carries AID* tag, empty sgRNA, and NATR marker

Depositor

Hiten Madhani

Insert

AID* - empty sgRNA - NATR

Tags

2xFLAG

Expression

Bacterial and Yeast

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDSW1728

Plasmid

#120812

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

Insert

red fluorescent protein (mCherry), codon-optimized for C. difficile

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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mOrange2-pBAD

Plasmid

#54531

Purpose

Localization: Protein Expression Vector , Excitation: 549, Emission: 565

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

6xHis and mOrange2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pHyo182

Plasmid

#173136

Purpose

Expresses pheS_A294G in e.coli

Depositor

Seokhee Kim

Article

Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

Insert

pheS_A294G

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAQ_G-alpha

Plasmid

#109809

Purpose

Protein expression and purification of GNAQ_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAQ_G-alpha (GNAQ Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Ezr-BioID2-HA

Plasmid

#176744

Purpose

Expresses Ezrin fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Ezr-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET14b His Rab8a Q67L

Plasmid

#186014

Purpose

His Rab8a Q67L

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

His Rab8a Q67L (RAB8A Human)

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Salmonella MTAN

Plasmid

#64041

Purpose

Expression of S. enterica MTAN in E. coli

Depositor

Vern Schramm

Article

Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

Insert

Methylthioadenosine nucleosidase

Tags

Histidine tag

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

Plasmid

#218509

Purpose

integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

mKate2

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Deletion of Plac_mrfp cassette, introduction of a…

Promoter

P23

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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