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PylRS-AS

Plasmid

#137908

Purpose

For incorporation of fluorophenylalanine derivatives

Depositor

Wenshe Liu

Publication

Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

Containing N346A, C348S mutations

Promoter

pBAD

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLib6.4

Plasmid

#133783

Purpose

Library backbone

Depositor

Norbert Perrimon

Publication

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SpyCatcher002-oTet

Plasmid

#124663

Purpose

Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

Depositor

Mark Howarth

Publication

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTet

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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OmpA-mNG-sfTq2ox

Plasmid

#124220

Purpose

Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

Depositor

Tanneke den Blaauwen

Publication

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

OmpA-mNG-sfTq2ox

Expression

Bacterial

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-eIF4E K119A

Plasmid

#112818

Purpose

Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

Depositor

Daniel Schoenberg

Publication

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Eif4e (Eif4e Mouse)

Tags

GST

Expression

Bacterial

Mutation

K119 mutated to A, increasing affinity for cap st…

Promoter

T7

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDD57

Plasmid

#119882

Purpose

Constitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Publication

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pConstitutive_GFP+

Expression

Bacterial

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDSG398

Plasmid

#115604

Purpose

surface-displayed Nb3; medium copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Mutation

See depositor comments below

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET41b+_Ufd1-His

Plasmid

#117107

Purpose

Production of recombinant Ufd1-His in E.coli

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

Ufd1 (UFD1 Human)

Tags

His

Expression

Bacterial

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDSG358

Plasmid

#115599

Purpose

surface-displayed Ag1; medium copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Mutation

See depositor comments below

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

Plasmid

#105234

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

Depositor

Kristala Prather

Publication

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pNS20-SpCas9-SNAP

Plasmid

#113717

Purpose

Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

Depositor

Gerald Schwank

Publication

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Publication

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Promoter

T7

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.1642

Plasmid

#108535

Purpose

CinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CinRAM + PCin-YFP

Expression

Bacterial

Mutation

CinR: S72C V80I G114D G115D R137H K220R A224T *24…

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSLIK CA RhoA

Plasmid

#112894

Purpose

promoter-inducible expression of constitutively-active RhoA

Depositor

Sanjay Kumar

Publication

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

CA RhoA (RHOA Human)

Use

Lentiviral

Expression

Bacterial

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK2-AraE

Plasmid

#110141

Purpose

Constitutive promoter expressing araE on RK2 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

araE

Expression

Bacterial

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCK306

Plasmid

#110544

Purpose

rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.

Depositor

John Heap

Publication

Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021

Inserts

PrhaBAD
yfp
rhaS

Expression

Bacterial

Promoter

N/A, PrhaBAD, and kanR promoter (upstream of kanR…

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)

Plasmid

#110558

Purpose

miniTn7 delivery plasmid with lacIq-Ptac inducible promoter

Depositor

Joanna Goldberg

Publication

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

lacIq-Ptac

Expression

Bacterial

Promoter

lacIq-Ptac

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Publication

Stephens et al Commun Biol. 2023 May 15;6(1):526. doi:

Insert

alpha synuclein (SNCA Human)

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSMART-SOS-GFPuv

Plasmid

#102283

Purpose

Fluorescent sensor of E. coli SOS response

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

cda-GFPuv

Expression

Bacterial

Promoter

cda SOS promoter

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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