32,963 results
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Plasmid#71742PurposeModular shuttle vector for Geobacillus and E. coliDepositorTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only
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pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
TagsFLAG, StrepII, and superfolder GFPExpressionBacterialPromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His10 TEV LIC cloning vector (2B-T-10)
Plasmid#78173DepositorTypeEmpty backboneTags10xHis and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK1
Plasmid#73248PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNatA (pACYCduet-naa10-naa15)
Plasmid#72928PurposeAllows expression of a modified version of the fission yeast NatA complex - chloramphenicol markerDepositorExpressionBacterialMutationCodon optomised for E.coli expressionPromoterT7Available SinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-neo
Plasmid#71320PurposeDonor plasmid - kanamycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPS22 (pET23a-Cas10/Csm/csm2H6N)
Plasmid#70038PurposeBacterial expression of the S. epidermidis Cas10·Csm complexDepositorInsertCas10/Csm complex
Tags6xHIS on N terminus of Csm2ExpressionBacterialPromoterT7Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM597
Plasmid#68886PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-tetR
Plasmid#62562PurposeTetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GCaMP6f
Plasmid#67564Purposebacterial expression of a fluorescent calcium reporterDepositorInsertGCaMP6f
TagsHIS-tagExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP
Plasmid#63215PurposeExpression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianPromoterCMV-EF1α hybrid (CEF)Available SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-E2-25K
Plasmid#63572PurposeExpression of human Ubiquitin conjugating enzyme E2-25KDepositorInsertUBE2-25K (UBA7 Human)
TagsGSTExpressionBacterialMutationIsoform 1 (200 resiudes)PromotertacAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b67Rb
Plasmid#58311PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.DepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTG-mTDG
Plasmid#52266PurposeBacterial expression of mouse TDG with C-terminal GST-tagDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI
Plasmid#50723Purposeoverexpression of human TPI1 in E.coliDepositorAvailable SinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-iRFP702
Plasmid#45455DepositorInsertiRFP702
ExpressionBacterialMutationT2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…PromoteraraBADAvailable SinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS-WT
Plasmid#44978DepositorAvailable SinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
BRD9
Plasmid#39012PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40731DepositorInsertAurora B FRET-sensor
TagsCyPet and YPetExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40571DepositorInsertAurora B FRET-sensor N-terminally fused to histone 2B
TagsCyPet and YPetExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only