30,260 results

1. pAG79-attL1-pegRNA-attL2

   Plasmid

   #213050

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   35S-CmYCLV-AtU6

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTn7-SCOUT11.R

   Plasmid

   #201003

   Purpose

   pLv1-pUC18R6KT-miniTn7-Gm-mCherry, GmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23104

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28c-F30-2xdBroccoli

   Plasmid

   #66843

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1-Zn2

   Plasmid

   #174805

   Purpose

   Bacterial expression of isolated PARP1 Zn2 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn2 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1-Zn3

   Plasmid

   #174806

   Purpose

   Bacterial expression of isolated PARP1 Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn3 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE30-His-IF3

   Plasmid

   #124125

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 3 (infC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FL MALT1 WT in pET29b-Strep

   Plasmid

   #48968

   Purpose

   Expresses Full length wild type MALT1 in e.coli

   Depositor

   Guy Salvesen

   Article

   Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

   Insert

   MALT1 isoform A (MALT1 Human)
   

   Use

   Tags

   His and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pYTK-DN2

   Plasmid

   #180283

   Purpose

   Spacer cassette (TU3)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConL2 - Spacer - ConRE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAraH6HATT

   Plasmid

   #63901

   Purpose

   For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

   Depositor

   Carlos Barbas

   Article

   Barbas et al Methods, A companion to Methods in Enzym

   Insert

   TT Fab
   

   Use

   Tags

   6xHis and HA tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFGL822

    Plasmid

    #58225

    Purpose

    For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

    Depositor

    Naweed Naqvi

    Article

    Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

    Insert

    phosphinothricin acetyl transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST14-DogCatcher

    Plasmid

    #171772

    Purpose

    Expresses DogCatcher protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. 560HTR

    Plasmid

    #24444

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pET GFP LIC cloning vector (u-msfGFP)

    Plasmid

    #29772

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    SiriusGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pET32a-HD25Q

    Plasmid

    #11508

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 25 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 25 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmRFP_LAP2beta_IRES_puro2b

    Plasmid

    #21047

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    LAP2_beta (TMPO Rat)
    

    Use

    Tags

    mRFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKD237-3a-2

    Plasmid

    #90957

    Purpose

    Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23100, Bba_J23105, and PphsA

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRBad-Z-CspGFP

    Plasmid

    #40730

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    Leucine Zipper prime - C super positive Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Use

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV, p10

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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