30,738 results

1. pBAD miniSOG

   Plasmid

   #91774

   Purpose

   Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   mini Singlet Oxygen Generator
   

   Tags

   Hexa-Histidine tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAB064d

   Plasmid

   #79197

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   Plac62; Ppro1

   Available Since

   Dec. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnEA-vH_His-TEV-SEPT2_SEPT6

   Plasmid

   #174497

   Purpose

   bacterial co-expression of human SEPT2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM4Z-OMA1

   Plasmid

   #61718

   Purpose

   human Oma1 protease in the E.coli/in vitro translation vector

   Depositor

   Oleh Khalimonchuk

   Insert

   OMA1 (OMA1 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pDonor-GDH

   Plasmid

   #140629

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   Mini-Tn6677, T7lac-GDH gene cassette
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Reb_1 aka PAS619

   Plasmid

   #71224

   Purpose

   Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA G53A

   Promoter

   T7

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pAD-LyseR

   Plasmid

   #99244

   Purpose

   For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Expression

   Bacterial

   Promoter

   beta-lactamase promoter

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTnsABC

   Plasmid

   #160031

   Purpose

   Expresses V. cholerae TnsABC

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTnsA, VchTnsB, VchTnsC
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   no

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. COVID-SARS2 NSP13

   Plasmid

   #159614

   Purpose

   Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

   Depositor

   Opher Gileadi

   Article

   Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

   Insert

   Covid-SARS2 Nsp13
   

   Tags

   His6-Zb-TEV

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   T7 - LacO

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule2-Acd82

    Plasmid

    #174100

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-AviTag-SnoopLigase

    Plasmid

    #105626

    Purpose

    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    AviTag-SnoopLigase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAT13araE

    Plasmid

    #18987

    Depositor

    Jay Keasling

    Article

    Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

    Insert

    araE (araE E.coli)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pET-PfuX7

    Plasmid

    #182364

    Purpose

    Expresses PfuX7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

    Insert

    PfuX7 DNA polymerase
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    V93Q

    Promoter

    T7

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBsVchCAST-pyrE

    Plasmid

    #203813

    Purpose

    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-mhYFP

    Plasmid

    #186532

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    araB

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGLO-GFP-1UAG

    Plasmid

    #82500

    Purpose

    Expresses GFP with 1 UAG codon at the amino acid position 3

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescent Protein with 1 UAG codon
    

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSP1

    Plasmid

    #20060

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1 (APOA1 synthetic gene)
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of deletion mutant (1-43) of human…

    Available Since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWP78-PVCpnf_pvc13-Ad5RGDPK7

    Plasmid

    #198291

    Purpose

    Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-Ad5RGDPK7
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGMC00018 (aka pRC0082)

    Plasmid

    #195320

    Purpose

    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial and Mammalian

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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