34,175 results
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Plasmid#199231PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpidermal growth factor, partial (EGF Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-sfGFP
Plasmid#199248PurposeFluorescent labelling of bacterial cells with sfGFPDepositorInsertCodon-optimized sfGFP gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-BrCas12b-RFND
Plasmid#195339PurposeExpresses Engineered BrCas12b in BacteriaDepositorInsertBrCas12b
UseCRISPR and Synthetic BiologyTags6xHis and 6xHis, MBP, TEV cleavage siteExpressionBacterialMutationR160E, F208W, N524V, D868VPromoterT7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEA186
Plasmid#187874PurposeEmpty pVS1-based INTEGRATE vector with mCherry cargoDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
L4440_BioBrick-SCR-sgRNA-A
Plasmid#177811PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
ExpressionBacterialAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike
Plasmid#173921PurposeExpresses the original SARS-Cov-2 Spike Protein under a CMV PromoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-2 Spike Protein (S Synthetic, SARS-COV-2, Human)
Tagsno tags, native proteinExpressionBacterial and MammalianPromoterstandard CMVAvailable sinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTCF156 HLA-A*02:01
Plasmid#180456PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-NLS-6xHis (gbr2101)
Plasmid#181743Purposebacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pATZ-Myc
Plasmid#177718PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc taggedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsMycExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCE27
Plasmid#174405PurposeC. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterialAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ALPaGA
Plasmid#175272PurposeLactate biosensor in anaerobic conditionsDepositorInsertLldR_Synthetic_promoter_sfGFP
ExpressionBacterialAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PRMT1-Halo
Plasmid#175334PurposeProduction of HaloTag fusion proteinDepositorPublicationAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterrrnB P1Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-AznLRS
Plasmid#172482PurposeFor E.Coli expression, 306L309L348I384F411WDepositorInsertAznLRS and pylTcua
ExpressionBacterialPromoteraraBADAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas12f1
Plasmid#171182PurposeConstitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1DepositorInsertCas12f1
UseCRISPRExpressionBacterialPromotercas9 promoter from Streptococcus pyogenesAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
TagsT7 tag and T7RNAPExpressionBacterialPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag
Plasmid#167269PurposeOverexpression of SNAP-tag in E. coliDepositorInsertSNAP-tag
TagsHis x10ExpressionBacterialPromoterT7Available sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX/PD-L1 WT
Plasmid#121466PurposeExpression of GST-human PD-L1 in E. ColiDepositorAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC57-Curlcake1
Plasmid#139340PurposeExpresses Curlcake1 (CC1) in E.coliDepositorInsertCurlcake1 with T7 promoter
ExpressionBacterialPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC57-Curlcake2
Plasmid#139341PurposeExpresses Curlcake2 (CC2) in E.coliDepositorInsertCurlcake2 with T7 promoter
ExpressionBacterialPromoterT7Available sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by