30,260 results

1. PIA900

   Plasmid

   #104401

   Purpose

   Polycistronic vector for expression of E. coli core RNAP

   Depositor

   Irina Artsimovitch

   Article

   Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

   Insert

   rpoA, B, C, and Z
   

   Use

   E. coli expression vector

   Tags

   encodes a C-terminally His-tagged rpoC which can …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115267

   Purpose

   Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   LbuCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGST-PSP-hTG

   Plasmid

   #85470

   Purpose

   bacterial expression of human tissue transglutaminase

   Depositor

   Ray Truant, Jeffrey Keillor

   Article

   Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

   Insert

   hTG2 (TGM2 Human)
   

   Use

   Tags

   GST and PSP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. rbcLS-pMAL-2px

   Plasmid

   #41621

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   rbcL/rbcS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Nov. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-Skp-TF

   Plasmid

   #83921

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Trigger Factor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTIII.EFGP.IGR3.6

   Plasmid

   #40866

   Depositor

   Harold Gainer

   Article

   Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

   Insert

   MOUSE OXYTOCIN GENE (Oxt Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   mouse oxytocin promoter

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWT019a

   Plasmid

   #107893

   Purpose

   W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-sd2U-Cas9, PLacO-sgRNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Use

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28a+GFP-CAMSAP3 CKK

   Plasmid

   #59033

   Purpose

   expresses CKK domain of CAMSAP3 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP3 CKK domain (CAMSAP3 Mouse)
   

   Use

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCRISPomyces-FnCpf1

    Plasmid

    #129554

    Purpose

    Contains codon-optimized FnCpf1 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    FnCpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HS69 pComb3xss anti-Homer1 nanobody

    Plasmid

    #134716

    Purpose

    Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))
    

    Use

    Tags

    6xHis-HA

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. His6TEV-UbG76C

    Plasmid

    #75299

    Purpose

    Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    human Ubiquitin mutant G76 to C
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Glycine 76 to Cystine

    Promoter

    T7

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP1337

    Plasmid

    #119270

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMW363

    Plasmid

    #238208

    Purpose

    Expresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    TwinStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMW364

    Plasmid

    #238205

    Purpose

    Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMAL-AGR2

    Plasmid

    #18097

    Depositor

    Ronny Drapkin

    Insert

    AGR2 (AGR2 Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pKEW302-AcrVA4

    Plasmid

    #115656

    Purpose

    Expresses AcrVA4 in bacteria, under TetR control.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKEW189-MBP-TEV-AcrVA1

    Plasmid

    #115660

    Purpose

    Expresses AcrVA1 in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA1
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSP1E2D1

    Plasmid

    #161606

    Purpose

    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E2D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge