30,738 results

1. pJYS2.1-crtYf

   Plasmid

   #89077

   Purpose

   Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYF
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Omega 1

   Plasmid

   #118046

   Purpose

   GoldenBraid2.0 Omega 1 destination vector

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Assembly of gb2.0 transcriptio…

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVSV eGFP RABV-G

   Plasmid

   #31833

   Depositor

   Connie Cepko

   Article

   Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   VSV genome
   

   Expression

   Bacterial

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSupAR-MbPylRS(DiZPK)

   Plasmid

   #91705

   Purpose

   express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

   Depositor

   P. Chen

   Article

   Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

   Inserts

   pylS
   pylT
   

   Expression

   Bacterial

   Mutation

   T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mH6-Cas12i2

   Plasmid

   #120883

   Purpose

   Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i2
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbAW4k-loxP-TT-loxP-mRFP1

   Plasmid

   #134405

   Purpose

   Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

   Depositor

   Mary Dunlop

   Article

   Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

   Insert

   W4-loxP-TT-loxP-mRFP1
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   W4 (modified T7A1)

   Available Since

   Jan. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGR

   Plasmid

   #46002

   Purpose

   Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose

   Depositor

   Christopher Voigt

   Article

   Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   June 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET mNG-GECO1

   Plasmid

   #201933

   Purpose

   Expresses mNG-GECO1 indicator in bacteria

   Depositor

   Robert Campbell

   Article

   Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

   Insert

   mNeonGreenGECO1
   

   Tags

   HIS tag

   Expression

   Bacterial

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28c-F30-2xdBroccoli

   Plasmid

   #66843

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET42b-AncBE4max

    Plasmid

    #165157

    Purpose

    Expresses NLS-AncBE4max-NLS with an N-terminal His Tag (6xHis) for bacterial expression

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    AncBE4max
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PT7lacO-EF-Tu-His8

    Plasmid

    #224370

    Purpose

    Express EF-Tu protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    EF-Tu (tufB E. coli)
    

    Tags

    His8

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b PKA Cat

    Plasmid

    #14921

    Depositor

    Susan Taylor

    Article

    Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

    Insert

    PKA catalytic subunit alpha (Prkaca Mouse)
    

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Available Since

    July 24, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJExpress414_GmPOPB-D661A

    Plasmid

    #92236

    Purpose

    Express D661A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-D661A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Aspartate 661 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWUR790

    Plasmid

    #101723

    Purpose

    Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

    Depositor

    John van der Oost

    Article

    Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    PfAgo
    

    Tags

    Strep(II)-tag

    Expression

    Bacterial

    Promoter

    T7 Promotor

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCasY1

    Plasmid

    #87687

    Purpose

    p15A plasmid expressing CasY1

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasY1
    

    Expression

    Bacterial

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVP144

    Plasmid

    #222081

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and eforRED
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBZS-YP_001497877

    Plasmid

    #198366

    Purpose

    Express YP_001497877 in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    YP_001497877 (B681L )
    

    Expression

    Bacterial

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJExpress414_GmPOPB-H698N

    Plasmid

    #92242

    Purpose

    Express H698N mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-H698N mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Histidine 698 to Asparagine

    Promoter

    T7 promoter

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCpf1b-sp

    Plasmid

    #122188

    Purpose

    Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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