30,416 results

1. tdTomato-pBAD

   Plasmid

   #54856

   Purpose

   Localization: Protein Expression Vector , Excitation: 554, Emission: 581

   Depositor

   Nathan Shaner, Michael Davidson, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   6xHis and tdTomato

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. HC-M

   Plasmid

   #173481

   Purpose

   An rrnB P1-based GFP ATP reporter in E. coli

   Depositor

   Rahul Sarpeshkar

   Article

   Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

   Insert

   GFP-mut2
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   rrnB P1

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSWAP_Entry_H2_beta_CM

   Plasmid

   #184870

   Purpose

   pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 2, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAIP1

   Plasmid

   #233685

   Purpose

   Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.

   Depositor

   Christopher Bahl

   Insert

   Ulp1-like SUMO protease
   

   Use

   Tags

   CAHS-107838 and HisTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. bdSUMO-GFP-HiBit

   Plasmid

   #170989

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-51b(+)_NAD-Snifit

   Plasmid

   #113658

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGRG25

   Plasmid

   #16665

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

   Insert

   mTn7::MCS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-FnCas12a-TEV

   Plasmid

   #174658

   Purpose

   FnCas12a expression plasmid

   Depositor

   Huimin Zhao, Jin Wang

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NowGFP /pQE-30

   Plasmid

   #74749

   Purpose

   NowGFP gene for bacterial expression

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

   Insert

   NowGFP
   

   Use

   Tags

   HisTag (with a mutation but still working)

   Expression

   Bacterial

   Mutation

   WasCFP with substitutions E6K, L42M, T43S, V68M, …

   Promoter

   T5

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK19pA-MN

    Plasmid

    #86973

    Purpose

    ChIC; genomic mapping of chromatin proteins

    Depositor

    Ulrich Laemmli

    Article

    Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

    Insert

    protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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