32,521 results
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Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only
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pKD280.7
Plasmid#173172PurposeExpresses SO_4387DepositorInsertSO_4387
ExpressionBacterialPromoterJ23107Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-MagLOV
Plasmid#219957PurposeBacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2DepositorInsertMagLOV
Tags6xHisExpressionBacterialMutationAsLOV2 C450P L496V Q513K G528K D540MPromoterT7Available SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pExp-Trx
Plasmid#112577PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-TrxA and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/611
Plasmid#26661DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIS001
Plasmid#194012PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertROSA-TRE-C16V5-G-pA
ExpressionBacterialAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCHERRY3
Plasmid#24659DepositorInsertmCherry
ExpressionBacterialAvailable SinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterNative promoter from pMUT2 relB/relE operon and P…Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA9-Halo
Plasmid#175332PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only