31,793 results
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Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only
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pKB01_tagRFP
Plasmid#69103PurposeExpresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInserttagRFP
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF184
Plasmid#89482PurposeExpress asparaginyl endopeptidase 1 from O. affinis (OaAEP1)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
TagsSmt3ExpressionBacterialPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGD248
Plasmid#219498PurposeTemperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)DepositorInsertEco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
ExpressionBacterialPromoterpBADAvailable SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
evoFERNY-Cas9NG
Plasmid#203329PurposePlasmid for bacterial purification of codon optimized evoFERNY-Cas9NGDepositorInsertevoFERNY-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB225
Plasmid#170015PurposeEncodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Peptide display protein with no C-terminal peptide
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pRSET-b
Plasmid#188446PurposeB-gTEMP for bacterial expressionDepositorInsertB-gTEMP
Tags6xHis-tagExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCascade(Ptr)-entry(BsaI)
Plasmid#175579PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-NDK
Plasmid#124136PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM2s2AsG
Plasmid#137924PurposeE. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expressionDepositorInsertSuperfolder GFP
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoAp-GFPuv
Plasmid#139072PurposeLow phosphate inducible promoter phoAp for GFP expressionDepositorInsertphoAp-GFPuv
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-AlaRS
Plasmid#124103PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAL-p5X
Plasmid#150814PurposeExpresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneTagsmalE A313VExpressionBacterialPromotertacAvailable SinceJuly 22, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMW363
Plasmid#238208PurposeExpresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpRDepositorInsertTaTasR
TagsTwinStrepIIExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYC-prk:nptII
Plasmid#239580PurposeSelection plasmid for Rubisco directed evolutionDepositorInsertPRK-NPTII
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
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His6_BcLOV4_Q355N_mCherry_BamUK
Plasmid#119762PurposeEncodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cellDepositorInsertBcLOV4-Q355N_mCherry
TagsmCherryExpressionBacterialMutationMutated glutamine at position 355 to asparaginePromoterT7Available SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PprgH-GFPLVA
Plasmid#23340DepositorInsertGFP(LVA)
TagsGFP(LVA)ExpressionBacterialAvailable SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLG007 (Ec86 retron)
Plasmid#157885PurposeExpresses the Ec86 retron defense systemDepositorInsertEc86 retron
ExpressionBacterialAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only