31,059 results

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pET28b(+)_rPD-L1nb

Plasmid

#226715

Purpose

Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag and Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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PGL4.11-COL1A1WT

Plasmid

#230915

Purpose

Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter (COL1A1 Human)

Use

Luciferase

Tags

luciferase - Luc2p

Expression

Bacterial and Mammalian

Promoter

COL1A1

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:fflucRT

Plasmid

#88901

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + Red-shifted firefly luciferase

Expression

Bacterial

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pB-Rex

Plasmid

#122134

Purpose

B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

B. subtilis Rex

Expression

Bacterial

Promoter

apFAB338

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDule-pCNF

Plasmid

#85494

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7FTrp)RS

Plasmid

#177310

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK-DN4

Plasmid

#180285

Purpose

KanR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - KanR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUdO2c

Plasmid

#210231

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-N

Plasmid

#30225

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with N terminus cleavage (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 20*-62

Available Since

May 25, 2011

Availability

Academic Institutions and Nonprofits only

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pNuc-cis

Plasmid

#202796

Purpose

RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

Depositor

David Edgell

Article

Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

Insert

TevSpCas9, sgRNA cassette, oriT, CenArsHis

Expression

Bacterial

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huTsCas12a

Plasmid

#174687

Purpose

Expresses 6xHis-MBP tagged TsCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huTsCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pR0306 CcaCas13b His6-TwinStrep-SUMO

Plasmid

#182687

Purpose

Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

CcaCas13b

Tags

SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pET9d-hnRNP-A1

Plasmid

#23026

Depositor

Douglas Black

Insert

hnRNPA1 (HNRNPA1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL1-pCMV7.1

Plasmid

#155019

Purpose

N-terminal 3xFLAG-tagged CUL1 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 1 (CUL1 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPP381

Plasmid

#158798

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-2

Tags

6xHis

Expression

Bacterial

Mutation

RuvC-inactivating mutation D394A

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBolux

Plasmid

#188109

Purpose

Encodes a promoter-less Bacteroides-optimized lux cassette

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/BH

Plasmid

#26556

Depositor

Perry Hackett

Insert

pT2/BH

Expression

Bacterial

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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