30,743 results
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Plasmid#193142PurposeClostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporterDepositorInsertCbe ZTP Riboswitch sfGFP
UseSynthetic BiologyExpressionBacterialMutationDeleted A26 to disable ligand-bindingPromoterJ23119Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Portal_PhiV1
Plasmid#188811PurposePortal protein from phage PhiV1DepositorInsertPortal
ExpressionBacterialAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Terminase_ST32
Plasmid#188850PurposeLarge terminase from phage ST32DepositorInsertTerminase
ExpressionBacterialAvailable SinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EpAvs1
Plasmid#188788PurposeAvs1 from Erwinia piriflorinigrans CFBP5888DepositorInsertEpAvs1
ExpressionBacterialPromoterblaAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHen2 Avi-tag-linker-p3
Plasmid#119819PurposeFor cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophageDepositorInsertlinker with Avi-tag and p3
TagsAvi-tagExpressionBacterialAvailable SinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-TFIIF
Plasmid#171082PurposeCo-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permissionDepositorTagsHisExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CH-GECO2.1
Plasmid#52099PurposemCherry-based Calcium indicatorsDepositorInsertCH-GECO2.1
Tags6 His tagExpressionBacterialPromoterpBAD promotorAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides
Plasmid#172506PurposeEncodes DisCas7-11 protein alone with a golden gate site for spacer cloningDepositorInsertDisCas7-11 bacterial expression vector with golden gate site for guides
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_NoMTS_pET28
Plasmid#34705DepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationEncodes residues 43–246, corresponding to full-le…PromoterT7Available SinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
p368_DG
Plasmid#234691PurposeGolden gate domesticated pET28b with 3C-His6DepositorTypeEmpty backboneTags3C-His6ExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA
Plasmid#164615PurposeExpresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.DepositorInsertRenilla luciferase
Tags24xMS2v5 (3'UTR) and SunTagExpressionBacterial and MammalianPromoterTetCMVAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 AC30_eGFP
Plasmid#82715PurposeExpresses a GFP-tagged recombinant adenylyl cyclase.DepositorTagsFLAG purification tag and eGFPExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFnCpf1GG
Plasmid#131010PurposeBackbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of FnCas12a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
ExpressionBacterialPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAB988
Plasmid#133920PurposeminiICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoterDepositorInsertminiICE-tet(M)cat(PC194)_PT7-GFP
UseSynthetic BiologyExpressionBacterialPromoterT7 promoterAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVV102
Plasmid#215308PurposeExpresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPTacAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV103
Plasmid#215309PurposeExpresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPtetAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only