30,416 results

1. pSerOTS-C1* (V70)

   Plasmid

   #188537

   Purpose

   Phosphoserine incorporation into recombinant proteins

   Depositor

   Jesse Rinehart

   Article

   Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

   Inserts

   pSerRS
   Ef-pSer
   tRNApSer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations resulting from laboratory evolution

   Promoter

   glnS*

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ACOT4A

   Plasmid

   #42449

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ACOT4A (ACOT4 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. p368_DG

   Plasmid

   #234691

   Purpose

   Golden gate domesticated pET28b with 3C-His6

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   3C-His6

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p344_DG

   Plasmid

   #234674

   Purpose

   Golden gate domesticated pET28b with His6-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His6-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. NylCK-TS_pET28a

   Plasmid

   #215423

   Purpose

   Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR58.6

   Plasmid

   #63176

   Purpose

   Expresses CcaR constitutively and sfGFP under the PcpcG2-172 promoter

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   CcaR
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T MDM2 WT

   Plasmid

   #16237

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-cerebody

   Plasmid

   #236738

   Purpose

   Protein overexpression of cerebody

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   cerebody
   

   Use

   Tags

   His, HA, FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p27-mVenus reporter (PB)

   Plasmid

   #190731

   Purpose

   The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   mVenus
   p27
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   p27-F62A-F64A

   Promoter

   EF1α

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-GST-BcPC-PLC

    Plasmid

    #224255

    Purpose

    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

    Depositor

    Chiaki Murakami

    Article

    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

    Insert

    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    

    Use

    Tags

    GST and HRV-3C (LEVLFQGP) site

    Expression

    Bacterial

    Mutation

    The gene of Bacillus cereus ATCC 14579 was used f…

    Promoter

    lac promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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