33,906 results
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Plasmid#84650PurposeGateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.DepositorTypeEmpty backboneTagsMBPExpressionBacterialPromotertacAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pScaf-7560.1
Plasmid#111406PurposepScaf phagemid with insert for generating DNA origami scaffold 7560 bases in lengthDepositorInsertpScaf-7560.1
ExpressionBacterialAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAJM.884
Plasmid#108536PurposeAcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.DepositorInsertAcuRAM + PAcu-YFP
ExpressionBacterialMutationAcuR: E80K R154H L202MAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAJM.683
Plasmid#108531PurposeBetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.DepositorInsertBetIAM + PBetI-YFP
ExpressionBacterialMutationBetI: S115IAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1 ATF1
Plasmid#106286PurposeExpresses ATF1 in Ecoli off a T7 promoterDepositorInsertATF1
ExpressionBacterialPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACYC-GFPM2
Plasmid#102791PurposeExpresses GFP under WT T7 promoterDepositorInsertGreen Fluorescent Protein
ExpressionBacterialAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
TagsHis tagExpressionBacterialPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_attP
Plasmid#110095PurposeVector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.DepositorTypeEmpty backboneTags3xFLAGExpressionBacterialAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBBR1k-J23107-GFPuv
Plasmid#106389PurposepBBR1k-GFPuv with Anderson promoter J23107 expressing lacIDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2-gfp
Plasmid#109290PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertGFP
ExpressionBacterialMutationWTPromoterKanamycinAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAB076i3
Plasmid#79198PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmMaple3-CAM
Plasmid#101148PurposeFluorescent protein for superresolution imagingDepositorInsertE. coli codon optimized mMaple3
ExpressionBacterialPromoterpBADAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
TagsmKate2ExpressionBacterialAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-PCNA
Plasmid#105977Purposeentry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNADepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1-mVenus ME
Plasmid#103989PurposePlasmid encoding mVenus MEDepositorInsertmVenus ME
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC029 - Lw2Cas13a from Leptotrichia wadei F0279
Plasmid#91919PurposeExpresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLw2Cas13a and guide expression cassette
UseCRISPRExpressionBacterialAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-CHC(1-1074)-His6
Plasmid#100748PurposeBacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.DepositorAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKL001
Plasmid#98917PurposeConstitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.DepositorInsertCaPR
ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only