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pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Article

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Tags

HIS/6x

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

Plasmid

#176745

Purpose

Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTu-His

Plasmid

#124127

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Tu (tufB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3-SYFP2

Plasmid

#189764

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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T4 Lysozyme WT

Plasmid

#18110

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Available Since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only

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WT USP7 catalytic

Plasmid

#227201

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 207-560

Promoter

T7-promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAV0279

Plasmid

#149688

Purpose

Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SUMO_Eu1-MBP

Tags

3xFLAG-tag and His14-tag

Expression

Bacterial

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGT-S1

Plasmid

#122582

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF-SeAvs3

Plasmid

#188805

Purpose

Codon optimized SeAvs3 with C-terminal His tag

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJB007

Plasmid

#98995

Purpose

Express FtsI tagged with tagRFP-t in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsI

Tags

TagRFP-T

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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HeLa full-length MLCK-GFP

Plasmid

#46316

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pHis APEX2-eIF4A1

Plasmid

#129645

Purpose

Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

eukaryotic translation initiation factor 4A1 (EIF4A1 Human)

Tags

6xHis and APEX2

Expression

Bacterial

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-CRAC version 1

Plasmid

#101245

Purpose

Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

Depositor

Yubin Zhou

Article

He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

Insert

LOV2 and STIM1 cytosolic fragment

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTOX3

Plasmid

#127448

Purpose

Encodes Tse2 toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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KE-009

Plasmid

#133441

Purpose

Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

Depositor

Gerhard Wagner

Insert

EIF4H (EIF4H Human)

Expression

Bacterial

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJC2283 - pET28-sfGFP-SRT-His6

Plasmid

#250248

Purpose

Recombinant production of sortase-tagged sfGFP.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

sfGFP

Tags

Sortase recognition site, 6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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A24M04 mIgM heavy chain-pFEEKW

Plasmid

#234809

Purpose

Express membrane form of IgM heavy chain containing the VH region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

Depositor

Susan Pierce

Article

Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

Insert

A24M04 membrane IgM heavy chain (IGHM Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EEK promoter

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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