30,260 results

1. pJMP3653

   Plasmid

   #222348

   Purpose

   Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMApra-Ptet2

   Plasmid

   #218361

   Purpose

   Empty backbone with tetR-PtetA expression system with two tetR operator sites

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDSG312

   Plasmid

   #115613

   Purpose

   surface-displayed Nb2, Arabinose-inducible; medium-copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. phage UbiC G3BP1-SNAP

   Plasmid

   #119949

   Purpose

   Lentiviral vector expressing G3BP1-SNAP fusion protein

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSR78

   Plasmid

   #90210

   Purpose

   Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

   Inserts

   CcaS
   Cph8
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRMC2-sec:msfGFP

   Plasmid

   #194914

   Purpose

   Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET41-MBP-t-A3A-t-His

   Plasmid

   #109231

   Purpose

   Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

   Depositor

   Rahul Kohli

   Article

   Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

   Insert

   human APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbE2a-RFP

   Plasmid

   #35322

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZE21-GFPaav

   Plasmid

   #26643

   Depositor

   Michael Elowitz

   Article

   Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

   Insert

   pZE21-GFPaav
   

   Use

   Tags

   GFPaav

   Expression

   Bacterial

   Mutation

   na

   Promoter

   Available since

   June 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTn7-SCOUT12.G

    Plasmid

    #201000

    Purpose

    pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMemoryArray

    Plasmid

    #60585

    Purpose

    An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    MemoryArray
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-MH6-RspCas13d

    Plasmid

    #108305

    Purpose

    Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    RspCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQF-lacZ

    Plasmid

    #48094

    Purpose

    Cumate-inducible expression of lacZ

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Insert

    E. coli lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1delZn2

    Plasmid

    #173935

    Purpose

    Bacterial expression of PARP1 lacking Zn2 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn2 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 97-213 (V762A)

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET_MBP-RGG-GFP-RGG

    Plasmid

    #124949

    Purpose

    Expression of MBP-RGG-GFP-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    EGFP, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPO1

    Plasmid

    #209975

    Purpose

    Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (256_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCS2-HA-GAAZFP-FokI-RR

    Plasmid

    #18754

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    zinc finger nuclease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    RNA expression vector for expressing the zinc fin…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEvoltac-EcW-TGA-h14

    Plasmid

    #218764

    Purpose

    encodes EcWRS-h14 and EcW-tRNA-TGA

    Depositor

    Abhishek Chatterjee

    Article

    Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

    Insert

    EcWRS-h14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S8A, V144G, V146C

    Promoter

    tacI

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST17-UbE2L3

    Plasmid

    #15795

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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