29,729 results

1. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172503

   Purpose

   Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-hnRNPA2_FL_WT

   Plasmid

   #98662

   Purpose

   Expresses MBP-tagged full length hnRNPA2

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGY53

   Plasmid

   #221709

   Purpose

   Empty Cas12a vector for multiplex gene editing in fungi

   Depositor

   Kyle Pomraning

   Article

   Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

   Type

   Empty backbone

   Use

   CRISPR; Aspergillus

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29b_1_anti-GFP-nanobody

   Plasmid

   #210394

   Purpose

   Express anti-GFP nanobody in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   1_anti-GFP-nanobody
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. LacI-pLacI-pII pm

   Plasmid

   #218099

   Purpose

   Phagemid that expresses LacI, and pII under the Lac promoter

   Depositor

   Kevin Esvelt

   Insert

   pII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLRD18

   Plasmid

   #208270

   Purpose

   Production of ent-isokaurene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   ptmT2
   osKSL6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLRD17

   Plasmid

   #208269

   Purpose

   Production of syn-stemod-13(17)-ene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   osCPS4
   osKSL11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLRD16

   Plasmid

   #208268

   Purpose

   Production of syn-stemar-13-ene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   osCPS4
   osKSL8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLRD13

   Plasmid

   #208265

   Purpose

   Production of ent-cassa-12,15-diene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   ptmT2
   osKSL7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLRD10

    Plasmid

    #208262

    Purpose

    Production of labda-7,13(16),14-triene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    grTPS2
    sclav_p0491
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLRD09

    Plasmid

    #208261

    Purpose

    Production of biformene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    cldB
    cldD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLRD05

    Plasmid

    #208259

    Purpose

    Production of isopimara-8,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    cldB
    sare_1287
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLRD01

    Plasmid

    #208257

    Purpose

    Production of terpentetriene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    cyc1
    cyc2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLRD15

    Plasmid

    #208267

    Purpose

    Production of ent-sandaracopimaradiene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    ptmT2
    osKSL10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRARE-MBP-DEST

    Plasmid

    #84650

    Purpose

    Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

    Depositor

    Neil Olszewski

    Article

    Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDiGc

    Plasmid

    #59322

    Purpose

    Inducible expression of dsRed and constitutive expression of GFP in bacteria

    Depositor

    David Holden, Sophie Helaine

    Article

    Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

    Inserts

    EGFP
    dsRed
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and rpsM

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. p005-RFP-strong

    Plasmid

    #108317

    Purpose

    Synthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    RFP under controlf of strong synthetic constitutive promoter pFAB4005
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4005

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28CLY3

    Plasmid

    #21762

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pRSET his-mCherry

    Plasmid

    #113552

    Purpose

    For bacterial expression and purification of mCherry

    Depositor

    Jeanne Stachowiak

    Article

    DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

    Insert

    his-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZE27MC3

    Plasmid

    #75464

    Purpose

    Plasmid for constitutive expression of mcherry-pdt3

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    mcherry-pdt#3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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