31,059 results

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MARS_pNIC-CTB10H

Plasmid

#153053

Purpose

Expression construct for producing Avi tagged Methionyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

MARS (MARS1 Human)

Tags

TEV-Avi-His10

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-G6

Plasmid

#194589

Purpose

Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

G6 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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CP2-TCC

Plasmid

#179679

Purpose

Complementary plasmid

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd2] T7 RNAP-TCC linker-degron

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMRS-XylS-dCas9

Plasmid

#220196

Purpose

mini-Tn7 dCas9 expression vector - XylS/pM promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

XylS/pM

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NlaCascade-NLS-6xHis

Plasmid

#180213

Purpose

Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas5-Cas8-Cas7

Tags

6XHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Article

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGT-Ah10

Plasmid

#122577

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2_SEPT6

Plasmid

#174497

Purpose

bacterial co-expression of human SEPT2 and of human SEPT6

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN2 (SEPTIN2 Human)
SEPTIN6 (SEPTIN6 Human)

Tags

His6-TEV

Expression

Bacterial

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pACEC3GH (Hyg)

Plasmid

#110084

Purpose

Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCre-SacB-zeo

Plasmid

#107706

Purpose

Expresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

ble (Zeo resistance)

Expression

Bacterial

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-6xHis

Expression

Bacterial

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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Pet28a-His-tag-BS2

Plasmid

#220347

Purpose

E. coli expression vector to purify BS2

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

His tag

Expression

Bacterial

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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6xHis-MBP-huMb3Cas12a

Plasmid

#174677

Purpose

Expresses 6xHis-MBP tagged Mb3Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMb3Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PARG-c002

Plasmid

#173107

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PARG (PARG Human)

Tags

mCerulean

Expression

Bacterial

Mutation

S448-T976

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDJSVT87

Plasmid

#109302

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

OmpA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET-His-Cre

Plasmid

#123128

Purpose

Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Cre recombinase

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pKL10-mRuby-iATPSnFR1.0 bb118

Plasmid

#158980

Purpose

Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mruby-iATPSNfR1.0

Tags

mruby3

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#102557

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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