30,260 results

1. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Jason Peters, Carol Gross, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA24N-ligase-NLS-cTF

   Plasmid

   #87746

   Purpose

   IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and NLS linker - cTF (chimeric transcriptio…

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET14-GFP15_Encap

   Plasmid

   #86407

   Purpose

   Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 15aa CLP tag
   Encapsulin
   

   Use

   Tags

   6x Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET GFP LIC cloning vector (2GFP-T)

   Plasmid

   #29716

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, SiriusGFP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnCS_SEPT7_SEPT9_i5-TEV-Strep

   Plasmid

   #174502

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i5

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a(+)-HIV RT-6xHis-RBS-prot

   Plasmid

   #159149

   Purpose

   expresses His-tagged HIV reverse transcriptase and untagged HIV protease

   Depositor

   Andrea Pauli

   Article

   Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

   Inserts

   Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
   Human immunodeficiency virus 1 (HIV-1) protease
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   HIV-1 group M/subtype B – BH10 strain

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. NylCK_pET28a

   Plasmid

   #215422

   Purpose

   Expression construct for NylCK gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCK
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVC-Ds-E1b:eGFP-Ds

   Plasmid

   #102417

   Purpose

   Fluorescent vector for screening enhancer elements in zebrafish

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   E1b:eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Zebrafish E1b minimal promoter

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET_RTX

   Plasmid

   #102787

   Purpose

   Expresses the Reverse Transcriptase gene

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter + lac operator

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUA66 PrpsL GFP

    Plasmid

    #105606

    Purpose

    Expresses GFP under PrpsL

    Depositor

    Pamela Silver

    Article

    Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrpsL

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23b-Casp9-His

    Plasmid

    #11829

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pGEX4T-1-Optimized UBE1L

    Plasmid

    #165099

    Purpose

    ecoli optimized expression of GST-fusion of human Ube1L

    Depositor

    Arthur Haas

    Insert

    UbeE1L (UBA7 Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. NADK

    Plasmid

    #28268

    Depositor

    Cheryl Arrowsmith

    Insert

    NADK:HPC0AC-F12:C216828 (NADK Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains residues 68-426.

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pSL0284 (pQCascade_entry)

    Plasmid

    #130635

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

    Plasmid

    #176742

    Purpose

    Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Aqp4-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-vioABECD

    Plasmid

    #66535

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV0279

    Plasmid

    #149688

    Purpose

    Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SUMO_Eu1-MBP
    

    Use

    Tags

    3xFLAG-tag and His14-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid

    #49518

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38S8-P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINS-USP21 (USP, aa 196-565)

    Plasmid

    #61585

    Purpose

    Expresses human USP21 (USP domain) in E. coli.

    Depositor

    David Komander

    Article

    Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

    Insert

    USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Deleted aa 1-195.

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbE7a-RFP

    Plasmid

    #35313

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge