30,260 results

1. pBbdCas9S_Psyn-sgRNA500

   Plasmid

   #149640

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET FLIPE-600n

   Plasmid

   #13537

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E WT
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. bactin:GFP-LAP2b

   Plasmid

   #105980

   Purpose

   transgenesis, ubiquitous expression

   Depositor

   Caren Norden

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   LAP2b
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA421-Cas9tr

   Plasmid

   #138709

   Purpose

   Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Insert

   tracrRNA-Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-TEV-EGFP-PRC1

   Plasmid

   #167540

   Purpose

   For expression of His6-TEV-EGFP-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Use

   Tags

   EGFP and His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-21a_FnCpf1_2xNLS_protein_expression

   Plasmid

   #124548

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnCpf1_2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-TetOminiCMV-SKM

   Plasmid

   #136551

   Purpose

   Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLG031 (upx)

   Plasmid

   #157909

   Purpose

   Expresses the upx defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   upx (putative PD-(D/E)xK family phosphodiesterase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRE-Tn7-141

   Plasmid

   #118557

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZE21/UBP1/ClpS_V65I

    Plasmid

    #98567

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains the V65I mutation that increases discrim…

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDULE-ABK

    Plasmid

    #49086

    Purpose

    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

    Depositor

    Peter Schultz

    Article

    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

    Inserts

    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L274M, C313A, Y349F

    Promoter

    GlnRS and lpp

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAJM.474

    Plasmid

    #108526

    Purpose

    LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LuxR + PLuxB-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    VanR: T49I A111V P179S

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-MSP1D1deltaH4H5

    Plasmid

    #71716

    Purpose

    Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGI3EM17

    Plasmid

    #210569

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIntegrase_3

    Plasmid

    #60575

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid

    #181781

    Purpose

    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHV739

    Plasmid

    #117477

    Purpose

    Expression of NMT2

    Depositor

    Richard Kahn

    Article

    Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

    Insert

    NMT2 (NMT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a - Ec-ClpX

    Plasmid

    #111516

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW211b5

    Plasmid

    #136927

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9(N)-NpuN
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    see manuscript

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pReporter_8

    Plasmid

    #60568

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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