30,744 results

1. pFCyR2

   Plasmid

   #167513

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymRAM-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PCymRC

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR348

   Plasmid

   #124713

   Purpose

   Expression of narX under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narX (narX )
   lacI (lacI )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   S193* - LacI is truncated and likely deactivated.…

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. IL13 promoter luciferase

   Plasmid

   #11784

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-13 promoter (IL13 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Available Since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. EHMT2

   Plasmid

   #25503

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EHMT2 (EHMT2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pMC_MS2_DNA_VLP

   Plasmid

   #179156

   Purpose

   Expression of maturation protein and his-tagged coat protein dimer from MS2 phage

   Depositor

   Paul Freemont

   Article

   Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. His-TEV-PRC1

   Plasmid

   #167539

   Purpose

   For expression of His6-TEV-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJM105: pTac RFP on pCM66T

   Plasmid

   #74746

   Purpose

   pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

   Depositor

   Mary Lidstrom

   Insert

   RFP (BioBrick part number BBa_E1010)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pTac

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC001 - huLshC2C2-MBP for bacterial expression

   Plasmid

   #79150

   Purpose

   Expresses human codon-optimized LshC2c2 for purification in E. coli

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   C2c2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP1E2D1

   Plasmid

   #161606

   Purpose

   Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E2D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-TS2126 RnlA-Strep

    Plasmid

    #159350

    Purpose

    Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

    Depositor

    Takashi Ito

    Article

    Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

    Insert

    TS2126 RNA ligase
    

    Tags

    His6 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

    Plasmid

    #47580

    Purpose

    Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Inserts

    MKK4-EE (MAP2K4 Human)
    MKK7a1-EE (Map2k7 Mouse)
    

    Expression

    Bacterial

    Mutation

    Lacks the first 36 residues of the RefSeq protein…

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pMJ826

    Plasmid

    #39316

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Histidine 840 to Alanine (H840A)

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAX2

    Plasmid

    #117398

    Purpose

    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJKR-O-mphR

    Plasmid

    #62570

    Purpose

    Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTrcHIS-7D12Gln13TAG

    Plasmid

    #125264

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    Gln13TAG 7D12
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-26b-NbVHH05-ALFA-His

    Plasmid

    #171569

    Purpose

    Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-ALFA-His (UBE2J1 Human)
    

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. IL-4 promoter luciferase

    Plasmid

    #12195

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    IL-4 promoter (Il4 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTU1-A-SP44-sfGFP

    Plasmid

    #163757

    Purpose

    Expresses Streptomyces codon-optimised sfGFP

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Promoter

    SP44

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRha-ABE7.10-SpCas9-NG

    Plasmid

    #170663

    Purpose

    Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

    Depositor

    David Liu

    Article

    Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

    Insert

    ABE7.10-SpCas9-NG
    

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Promoter

    pRhaBAD

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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