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32,120 results

Showing: 6681 - 6720 of 32120 results

  6. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  7. TH

    Plasmid
    #39079
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TH (TH Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pNDGG003

    Plasmid
    #231316
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231316-plasmid-map-sequence-id-463764

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  13. pOPS0379

    Plasmid
    #115510
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115510-plasmid-map-sequence-id-224287

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  16. NUDT3A

    Plasmid
    #42348
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT3A (NUDT3 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pBAD_dGFPmut3-AAV

    Plasmid
    #187379
    Purpose
    arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187379-plasmid-map-sequence-id-370386

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  20. CLK1

    Plasmid
    #39149
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CLK1 (CLK1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39149-plasmid-map-sequence-id-160536

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  21. pQE-BR1

    Plasmid
    #160333
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    BR1
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160333-plasmid-map-sequence-id-326604

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  26. pDM053

    Plasmid
    #216815
    Purpose
    Aspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mNeonGreen
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216815-plasmid-map-sequence-id-429382

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  27. pDM071

    Plasmid
    #216817
    Purpose
    Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mCardinal
    Tags
    RIPGLIN
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216817-plasmid-map-sequence-id-430145

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  28. human KANK2 Ankyrin Repeats pET151

    Plasmid
    #164054
    Purpose
    Expresses human KANK2 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164054-plasmid-map-sequence-id-327671

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  33. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  34. pDLxR6

    Plasmid
    #172560
    Purpose
    Inducible gene expression, pCDF origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-mRFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLuxB
    Available Since
    Nov. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    172560-plasmid-map-sequence-id-347035

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  37. pET22-Nsp10-TEV-6His

    Plasmid
    #154957
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    Tags
    TEV-6His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154957-plasmid-map-sequence-id-306327

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  39. pEXP5-NT/pT7 LasI

    Plasmid
    #193631
    Purpose
    Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LasI
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193631-plasmid-map-sequence-id-382852

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Showing: 6681 - 6720 of 32120 results