31,059 results
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Plasmid#92101PurposeExpresses GST fusion PHD and Bromodomain from BPTF proteinDepositorAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only
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pMRE-Tn5-152
Plasmid#118534PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK066
Plasmid#65173PurposeEncodes tTDH1 as a Type 4b part to be used in the Dueber YTK systemDepositorInserttTDH1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeE
Plasmid#129120PurposepBADHISTEV carrying pfeE for overexpression of PfeE in E. coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removedPromoterPBADAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIRT6
Plasmid#28271DepositorAvailable SinceFeb. 10, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28HistevTtTERT(1-516)
Plasmid#28171DepositorInsertTetrahymena TERT
Tags6xhistidineExpressionBacterialAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAR100
Plasmid#180377PurposepAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).DepositorInsertsNFAT-Luciferase
hCD8A
ExpressionBacterial and MammalianPromoterhEF1-HTLV and minimal promoter and an NFAT respon…Available SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAMK2A
Plasmid#38971PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nck
Plasmid#186133PurposeFor expression of recombinant Nck in bacteria cells.DepositorInsertNCK1 (NCK1 Human)
TagsGSTExpressionBacterialMutationAmino acid residues 1-377, with mutations: C139S,…PromoterLacAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gfp-zf-vasa
Plasmid#167323PurposePlasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.DepositorInsertzf vasa 3'UTRs (ddx4 Zebrafish)
ExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF-BetI
Plasmid#49357DepositorInsertBetI repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTE-28S15-0X
Plasmid#20319DepositorInsertPsmyc-tetR#28
ExpressionBacterialAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …Available SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE154
Plasmid#118504Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmOrange2
ExpressionBacterialAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCPP5154
Plasmid#128704PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertavrPto
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gi-c010
Plasmid#173080PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGAP
TagsmCitrineExpressionBacterialMutationmCitrine + last 10 residues of GNA1, codon optimi…Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144B (27-236)
Plasmid#110753PurposeBacterial Expression of RNF144B RBR domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNM262
Plasmid#128412PurposeVanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseSynthetic BiologyTagsamyE SPExpressionBacterialPromoterPspank(V)Available SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/hGGA3(GAT)
Plasmid#79436PurposeExpresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coliDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSA-C
Plasmid#17329DepositorInsertstreptavidin
ExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…Available SinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-PTS
Plasmid#108953Purposeheterologous expression of PTS of P. cablin in E. coliDepositorInsertPatchoulol synthase (PTS)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETSG
Plasmid#159418PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coliDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-PAmCherry-KanMX6
Plasmid#87026PurposeTag S. pombe genes in C terminal with PAmCherryDepositorInsertPAmCherry
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-134
Plasmid#118520PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-MAP
Plasmid#66999PurposeExpresses malE-MAP in E. coliDepositorInsertmalE-MAP
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBacPuroR-NeoR
Plasmid#34921DepositorInsertsBacterial promoter::PuroR::bacterial 3p flank
Bacterial promoter::NeoR::bacterial 3p flank
ExpressionBacterialAvailable SinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAH121
Plasmid#164901PurposeThe pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.DepositorInsertphi21 Integrase
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP3416
Plasmid#128720PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopT1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5534
Plasmid#128723PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopX1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Rnl2(1-249)K227Q
Plasmid#14072DepositorInsertRnl2(1-249)
TagsHis (10x)ExpressionBacterialMutationchanged Lys227 to Glutamine Gly112 is actually C…Available SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKERep
Plasmid#211826PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MZB174
Plasmid#228487Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneTags10xHis-SUMO(Smt3)-GST-HRVExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPPC019.AAV
Plasmid#171147PurposeExpression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmidDepositorInsertshAAVS1 scRNA
J1-BBa_J23117-mRFP
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J1-BBa_J23117Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
TagsmClover3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-WT-N’His
Plasmid#117701PurposeExpression of WT mouse SOD1 with N- terminal His tagDepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-BRT17
Plasmid#45358DepositorInsert17 Repeat Beta Roll Tag
TagsMaltose Binding Protein (MBP)ExpressionBacterialPromoterPtacAvailable SinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only