30,261 results

1. pVoPo-mNG

   Plasmid

   #187843

   Purpose

   Constitutive mNeonGreen-expression vector for Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b_PstS_2

   Plasmid

   #78199

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C; A197C (please see depositor comment below)

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-43_CTD_Q331K

   Plasmid

   #98673

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Q331K

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE60 MGH

   Plasmid

   #204244

   Purpose

   Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MGH
   

   Use

   Tags

   6X His and GFP

   Expression

   Bacterial

   Mutation

   fusion of MDH and GFP with His tag

   Promoter

   Lac

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCKan-yibDp-mCherry

   Plasmid

   #127058

   Purpose

   Low phosphate inducible mCherry with N-terminal linker and His tag

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE81XN-mStayGold(J)

   Plasmid

   #225445

   Purpose

   Expresses Hisx6-Tagged mStayGold(J) in bacteria

   Depositor

   Benjamin Glick

   Insert

   mStayGold(J)
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_GNAQ_G-alpha

   Plasmid

   #109809

   Purpose

   Protein expression and purification of GNAQ_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAQ_G-alpha (GNAQ Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-2/Rabaptin-5:R5BD

   Plasmid

   #168808

   Purpose

   Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

   Depositor

   Guangpu Li

   Article

   Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

   Insert

   Rab5-binding domain (aa739-862) of Rabaptin-5
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

   Plasmid

   #153526

   Purpose

   Expression of the anti SARS-CoV-2_RBD sybody Sb#45

   Depositor

   Markus Seeger

   Article

   Walter et al bioRxiv 2020.04.16.045419

   Insert

   Sb#45
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCpf1

    Plasmid

    #122185

    Purpose

    Bacterial genome editing plasmid with kanamycin resistance marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET42b-ABE7.10

    Plasmid

    #120398

    Purpose

    Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    Junjiu Huang

    Article

    Liang et al Nat Commun. 2019 Jan 8;10(1):67. doi: 10

    Insert

    ABE7.10
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mammalian codon-optimized

    Promoter

    T7

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-26b-Nb127D01-ALFA-His

    Plasmid

    #171568

    Purpose

    Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-ALFA-His (CXCR2 Human)
    

    Use

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. ILF2_DZF_pGEX

    Plasmid

    #135162

    Purpose

    Recombinant expression of ILF2 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    ILF2 (ILF2 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. mu-GM-CSF-pCIneo

    Plasmid

    #163518

    Purpose

    Expresses murine GM-CSF in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine GM-CSF
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC57-Curlcake1

    Plasmid

    #139340

    Purpose

    Expresses Curlcake1 (CC1) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake1 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC57-Curlcake2

    Plasmid

    #139341

    Purpose

    Expresses Curlcake2 (CC2) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake2 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC57-Curlcake4

    Plasmid

    #139343

    Purpose

    Expresses Curlcake4 (CC4) in E.coli

    Depositor

    Eva Maria Novoa

    Article

    Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

    Insert

    Curlcake4 with T7 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQE-60NA-proinsulin-msGFP2

    Plasmid

    #160466

    Purpose

    Encodes a fusion of human proinsulin and the monomeric superfolder GFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    proinsulin-msGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNR230

    Plasmid

    #79546

    Purpose

    BxbI attB-attP. GFP will express if sites recombine.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnCS_SEPT7_SEPT9_i3-TEV-Strep

    Plasmid

    #174501

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i3

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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