30,744 results

1. pXT130_TwinStrep-SUMO-ShTnsC

   Plasmid

   #135526

   Purpose

   Protein expression construct for ShTnsC

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsC
   

   Tags

   TwinStrep-SUMO

   Expression

   Bacterial

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTOX4

   Plasmid

   #127449

   Purpose

   Encodes YhaV toxin and amilCP chromoprotein. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Inserts

   yhaV toxin
   amilCP chromoprotein
   

   Expression

   Bacterial

   Mutation

   Codon optimized from protein sequence in FPbase

   Promoter

   pRha and tac

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TGT-His

   Plasmid

   #138201

   Purpose

   Expresses E. Coli TGT with a C-terminal His Tag

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Insert

   E Coli tRNA Guanine Transglycosylase-His
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-26b-Nb127D01-HA-His

   Plasmid

   #171566

   Purpose

   Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-HA-His (CXCR2 Human)
   

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. R6K_tac_mCherry_ChInt

   Plasmid

   #187382

   Purpose

   chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherry

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTOX8

   Plasmid

   #127453

   Purpose

   Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Inserts

   mqsR toxin
   tsPurple chromoprotein
   

   Expression

   Bacterial

   Mutation

   Codon optimized from protein sequence in FPbase

   Promoter

   apFAB46 and pRha

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX6P1-HA-MEK1

   Plasmid

   #53159

   Purpose

   Expresses GST-HA tagged human MEK1 from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Tags

   GST and HA

   Expression

   Bacterial

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDual-eGFP

   Plasmid

   #63215

   Purpose

   Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-MSP1D1deltaH4-H6

   Plasmid

   #71717

   Purpose

   Membrane scaffold protein (MSP), helix 4 to 6 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IL2 pMalLP

    Plasmid

    #41808

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Tags

    3C protease site, 6x His, and Maltose Binding Pro…

    Expression

    Bacterial

    Promoter

    pTac

    Available Since

    Jan. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-LacI

    Plasmid

    #46394

    Purpose

    Overproduction of LacI

    Depositor

    Florian Hollfelder

    Article

    Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

    Insert

    LacI
    

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    silent T to C mutation at position 19

    Promoter

    arabinose promoter

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-29b(+)_His-CBD-3C-EmPNGaseF

    Plasmid

    #218193

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

    Depositor

    Florence Pojer

    Insert

    PNGaseF
    

    Tags

    tufA-His-CBD-3C

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-354

    Promoter

    T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-His6-Ubc9

    Plasmid

    #133909

    Purpose

    Bacterial expression of His6-Ubc9

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTOX2

    Plasmid

    #127447

    Purpose

    Encodes MqsR toxin. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Insert

    mqsR toxin
    

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28A-Rab8A

    Plasmid

    #228880

    Purpose

    Expression of His-tagged Rab8A in E. coli

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    RAB8A (RAB8A Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    residues 1-6 and 177-207 missing

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET30a_HIV-RT

    Plasmid

    #153311

    Purpose

    Bacterial expression of HIV reverse transcriptase

    Depositor

    Bryan Dickinson, Drew Endy

    Insert

    HIV Reverse Transcriptase
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGEX-4T-3-mR7BD

    Plasmid

    #79149

    Purpose

    bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

    Depositor

    Aimee Edinger

    Article

    Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

    Insert

    R7BD (Rilp Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 220-299

    Promoter

    tac

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-AR-LBD

    Plasmid

    #171239

    Purpose

    Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL403

    Plasmid

    #119953

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid

    #196182

    Purpose

    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Inserts

    GSK3B (GSK3B Human)
    λPPase
    

    Tags

    GST, HA, His6, and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge