30,749 results

1. eNS2B47NS3 L30SF31S

   Plasmid

   #155319

   Purpose

   DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   L30 to S, F31 to S

   Promoter

   M13

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RepX_pET28a(+)

   Plasmid

   #172199

   Purpose

   Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

   Depositor

   Taekjip Ha

   Article

   Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

   Insert

   RepX superhelicase
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   C18L, C43S, C167V, S400C, C612A

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET23b-Casp9-His

   Plasmid

   #11829

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

   Insert

   Caspase 9 (CASP9 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pEB1030

   Plasmid

   #22067

   Depositor

   Emmanuelle Bouveret

   Article

   Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

   Type

   Empty backbone

   Tags

   T18-Flag

   Expression

   Bacterial

   Available Since

   Oct. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUCSh5vio-2300

   Plasmid

   #170987

   Purpose

   Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300 violacein
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   placI

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p3993 pET ubcH7

   Plasmid

   #8654

   Depositor

   Peter Howley

   Article

   Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

   Insert

   UbcH7 (UBE2L3 Human)
   

   Expression

   Bacterial

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pRha-ABE8e-NRCH

   Plasmid

   #165417

   Purpose

   For bacterial purification of ABE8e-NRCH protein

   Depositor

   David Liu

   Insert

   ABE8e-NRCH
   

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Promoter

   pRhaBAD

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-MH6-RspCas13d_RspCasWYL1

   Plasmid

   #108304

   Purpose

   Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Inserts

   RspCas13d
   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET23b-Casp3 C163A-His

   Plasmid

   #11822

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 3 catalytic mutant (CASP3 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C163A

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pEGFPN1-human Dynamin 1aa

    Plasmid

    #22163

    Depositor

    Pietro De Camilli

    Article

    Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

    Insert

    human Dynamin 1aa (DNM1 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    D744N

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJMP12042

    Plasmid

    #222354

    Purpose

    Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLJSRSF3

    Plasmid

    #64692

    Purpose

    His-GST-Smt3-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, GST, HisTag, NpuDnaE-N102, and Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMyC-kan

    Plasmid

    #84692

    Purpose

    Protein expression in mycobacteria

    Depositor

    Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    Acetamidase

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC18T-mini-Tn7T-hph-dCas9

    Plasmid

    #218371

    Purpose

    Expression of dCas9 for CRISPR-interference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PtetA

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETduet+sfGFP-TetR-p300HAT

    Plasmid

    #157795

    Purpose

    Expression of sfGFP-TetR-p300HAT fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR-p300 (EP300 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 6798 pJC25NMstop

    Plasmid

    #1228

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    NM (SUP35 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. NLS-SpCas9mSA-His

    Plasmid

    #182951

    Purpose

    Express SpCas9mSA in bacteria

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    NLS-SpCas9mSA-His
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pL0M-S-mKOκ-EC18154

    Plasmid

    #137077

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYUB1062-GFP

    Plasmid

    #117695

    Purpose

    Express recombinant proteins with GFP-His fusion in Mycobacterium smegmatis

    Depositor

    Elizabeth Fullam

    Article

    Radhakrishnan et al RSC Adv. 2018 Sep 27;8(58):33087-33095.

    Type

    Empty backbone

    Tags

    GFP and His

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pProEx N-Ras(G12C) CL

    Plasmid

    #224262

    Purpose

    Protein purification of N-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    N-Ras (NRAS Human)
    

    Expression

    Bacterial

    Mutation

    G12C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge