29,744 results

1. pBad-mKalama1

   Plasmid

   #14892

   Purpose

   Bacterial expression of mKalama1 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 Apr 20. ():.

   Insert

   mKalama1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

2. GST-thr-RAF1-RBD

   Plasmid

   #86033

   Purpose

   Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

   Depositor

   Dominic Esposito

   Insert

   RAF1 RBD (Ras-binding domain) (RAF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains residues 1-149

   Promoter

   T7

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

   Plasmid

   #136627

   Purpose

   Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-LAMA-R59
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138130

   Purpose

   Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1354

   Plasmid

   #119275

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pin1

   Plasmid

   #40773

   Depositor

   Dustin Maly

   Insert

   Pin1 (PIN1 Human)
   

   Use

   Tags

   6His and TEV

   Expression

   Bacterial

   Mutation

   Starting Met removed

   Promoter

   T7

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJH65

   Plasmid

   #67273

   Purpose

   Conjugally transferable red recombinogenic plasmid

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA
   

   Use

   Red recombinase, temperature sensitive

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. KLHL7

   Plasmid

   #39023

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KLHL7 (KLHL7 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pTrc99A-Rat FABP1

    Plasmid

    #13577

    Depositor

    Martin Scanlon

    Article

    Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

    Insert

    Rat liver fatty acid binding protein (Fabp1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

    Plasmid

    #204816

    Purpose

    Bacterial expresison of Enterovirus A71 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    EV A71 3C protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Tol2-lyzC-mcherry-2a-CDK2

    Plasmid

    #121131

    Purpose

    neutrophil specific expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-WT CDK2 (cdk2 Zebrafish)
    

    Use

    Tags

    mcherry-2a

    Expression

    Bacterial

    Mutation

    Promoter

    neutrophil specific lyzC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET32a-HD16Q

    Plasmid

    #11487

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 16 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 16 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-HA-human OCRL

    Plasmid

    #22207

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Oculocerebrorenal syndrome of Lowe (OCRL Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. MICU-APEX2

    Plasmid

    #79057

    Purpose

    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    C-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.R_TRE_minPro

    Plasmid

    #211517

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SV40 and minPro

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE100

    Plasmid

    #59395

    Purpose

    MethyloBrick cloning vector, no promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    Oct. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. TccCas13a Twin SUMO

    Plasmid

    #199754

    Purpose

    Expresses codon optimized thermostabile TccCas13a in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    TccCas13a
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAJT194

    Plasmid

    #202293

    Purpose

    Type IIS cloning plasmid for LPattB5 integrations w/ OriT

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-EIN-AlkBH5(66-292)

    Plasmid

    #125732

    Purpose

    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    AlkBH5(66-292) (ALKBH5 Human)
    

    Use

    Tags

    6xHis and EIN

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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