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pDZ617 pKAN 12xPP7 V4

Plasmid

#72237

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Publication

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

12xPP7 V4

Use

Cre/Lox

Expression

Bacterial

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUCP20T-mko1

Plasmid

#78467

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Publication

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

mKO1

Expression

Bacterial

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-HpaBC

Plasmid

#71260

Purpose

Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

Depositor

Mattheos Koffas

Publication

Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

Inserts

HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE

Plasmid

#73438

Purpose

Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

Depositor

Mattheos Koffas

Publication

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6 (orthogonal T7-lac variant)

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pU6T7G

Plasmid

#71463

Purpose

Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

Depositor

Willie Mark

Publication

Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

human U6/phage T7

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJK635

Plasmid

#72473

Purpose

pET28a modified to contain TEV protease fusion tag instead of T7 tag

Depositor

T. J. Kappock

Type

Empty backbone

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJDC181

Plasmid

#60853

Purpose

Expresses click beetle red luciferase

Depositor

Jeffrey Cirillo

Publication

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Click beetle red luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Zeo-eyfp

Plasmid

#65039

Purpose

ZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

eyfp

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-GW

Plasmid

#64961

Purpose

mini-Tn7 Gateway destination vector

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMM660

Plasmid

#68889

Purpose

Arabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Cdc42 G15A

Plasmid

#69356

Purpose

expression of nucleotide free Cdc42 in bacterial cells

Depositor

Rafael Garcia-Mata

Publication

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pFCM1

Plasmid

#64948

Purpose

source of CmR FRT cassette

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-cat-FRT

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Publication

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)

Tags

Histidine tag and TEV

Expression

Bacterial

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Publication

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFGL821

Plasmid

#58223

Purpose

For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

Depositor

Naweed Naqvi

Publication

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

hygromycin B phosphotransferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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H6-auxilin

Plasmid

#62571

Purpose

6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria

Depositor

Ernst Ungewickell

Publication

Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304

Insert

Auxilin

Tags

6XHis

Expression

Bacterial

Mutation

*see comment below

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
H6-AP180

Plasmid

#63006

Purpose

expresses the adaptor protein AP180 in bacteria

Depositor

Ernst Ungewickell

Publication

Norris et al J Biol Chem. 1995 Jan 6;270(1):214-7.

Insert

AP180 (Snap91 Rat)

Tags

His-tag

Expression

Bacterial

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMemoryArray

Plasmid

#60585

Purpose

An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

MemoryArray

Expression

Bacterial

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTE101

Plasmid

#59396

Purpose

MethyloBrick cloning vector, no promoter; KmR

Depositor

Tobias Erb

Publication

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

none

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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