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pJMP2

Plasmid

#79874

Purpose

Bacillus subtilis sgRNA expression vector; integrates into amyE

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCri-1b

Plasmid

#61327

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MBP-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET28b-BE3∆UGI

Plasmid

#96943

Purpose

The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

Depositor

Daesik Kim

Article

Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

Insert

BE3∆UGI

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PS-Grb2 (XSB363)

Plasmid

#78505

Purpose

Construct for expressing Grb2 in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-Etanercept

Plasmid

#182421

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-Etanercept (TNFRSF1B Human)

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUCP20T-ecfp

Plasmid

#78463

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eCFP

Expression

Bacterial

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-DsRed-Express2

Plasmid

#111251

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-3

Plasmid

#172769

Purpose

Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pQLink-GST-10xCTD

Plasmid

#138472

Purpose

Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

10x CTD repeat

Promoter

T5

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pEP PolI

Plasmid

#11722

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I EP (polA E. coli)

Expression

Bacterial

Mutation

D424A, I709N, A759R. Error-prone version of PolI.

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pS24·Pm-GFP

Plasmid

#122594

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGL403

Plasmid

#119953

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PEBP1-Halo

Plasmid

#175333

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PEBP1 (PEBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pShHELIX_sgRNA_entry (CJT32)

Plasmid

#181782

Purpose

Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_SNX18-1/1

Plasmid

#91369

Purpose

Protein expression and purification of human SH3 domain construct SNX18-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX18-1/1 (SNX18 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-Top2A-5X Gly-Venus-T2A-Hygro

Plasmid

#211115

Purpose

For tagging the C-term of Top2A with Venus

Depositor

Job Dekker

Article

Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

Inserts

5' C-term Top2A homology arm (TOP2A Human)
Venus
T2A
Blastocidin Resistance
5' C-term Top2A homology arm (TOP2A Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

n/a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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TS181_pETNKI_his3C_pA-Dam

Plasmid

#200721

Purpose

producing pA-DamID methylation protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

His3C-pA-Dam

Tags

His

Expression

Bacterial

Mutation

codon optimized

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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Hela kinase-dead MLCK-GFP

Plasmid

#46317

Depositor

Anne Bresnick

Article

Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

Insert

HeLa long myosin light chain kinase-dead (MYLK Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

E1626K

Promoter

CMV

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only