30,753 results

1. QUAS-10-T7-GFP_ColE1

   Plasmid

   #171654

   Purpose

   QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-10-T7
   

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. QUAS-15-T7-GFP_ColE1

   Plasmid

   #171655

   Purpose

   QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-15-T7
   

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIM1773-PRHIII-sfGFP-pNW33N

   Plasmid

   #52217

   Purpose

   Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

   Depositor

   Ichiro Matsumura

   Article

   Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

   Insert

   PRHIII-sfGFP
   

   Expression

   Bacterial

   Promoter

   PRHIII

   Available Since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB1A3_GFP_dT17

   Plasmid

   #89660

   Purpose

   expression of doubleTAL staple protein dT17 with N-terminally fused cycle3-GFP

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   GFP_dT17
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP114

   Plasmid

   #120799

   Purpose

   E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

   Depositor

   Craig Ellermeier, David S. Weiss

   Article

   Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

   Insert

   Pxyl::mCherryOpt
   

   Expression

   Bacterial

   Mutation

   1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

   Promoter

   Pxyl

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P2-p53 (1-90) (72R)

   Plasmid

   #62081

   Purpose

   Expression of human p53 (TAD residues 1-90) (72R) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains TAD/PP residues 1-90, 72R

   Promoter

   tac

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

8. mOrange-pBAD

   Plasmid

   #54751

   Purpose

   Localization: Protein Expression Vector , Excitation: 548, Emission: 562

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   6xHis and mOrange

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB179-BhrPETase

   Plasmid

   #218692

   Purpose

   Encodes codon optimized BhrPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   BhrPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mu-FLT3Lv1-pTRIP

    Plasmid

    #163519

    Purpose

    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine-FLT3L variant 1 (Flt3l Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-K-GECO1

    Plasmid

    #105864

    Purpose

    Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Tags

    6-His Tag

    Expression

    Bacterial

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLG027 (qatABCD)

    Plasmid

    #157905

    Purpose

    Expresses the qatABCD defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    qatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGM1192

    Plasmid

    #115683

    Purpose

    E. coli-Streptomyces shuttle vector for gene expression

    Depositor

    Günther Muth

    Insert

    mCherry, aac(3)IV, tsr
    

    Tags

    Strep-tagII on mCherry, codon optimized

    Expression

    Bacterial

    Mutation

    mCherry codon optimized for Streptomyces

    Promoter

    SF14*

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-TEX264-deltaLRR-His6

    Plasmid

    #220215

    Purpose

    Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    c.4-75del / p.2-24del

    Promoter

    T7

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PIA900

    Plasmid

    #104401

    Purpose

    Polycistronic vector for expression of E. coli core RNAP

    Depositor

    Irina Artsimovitch

    Article

    Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

    Insert

    rpoA, B, C, and Z
    

    Use

    E. coli expression vector

    Tags

    encodes a C-terminally His-tagged rpoC which can …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid

    #199781

    Purpose

    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

    Depositor

    Sascha Martens

    Insert

    NEMO (IKBKG Human)
    

    Tags

    GST-Thrombin-TEV-EGFP

    Expression

    Bacterial

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHCKan-yibDp-GFPuv

    Plasmid

    #127078

    Purpose

    Low phosphate inducible yibD gene promoter expressing GFPuv reporter

    Depositor

    Michael Lynch

    Article

    Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSB1A3_GFP_dT21

    Plasmid

    #89662

    Purpose

    expression of doubleTAL staple protein dT21 with N-terminally fused cycle3-GFP

    Depositor

    Hendrik Dietz

    Article

    Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

    Insert

    GFP_dT21
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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