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pET28a-Ec.coaA

Plasmid

#50386

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss

Article

Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

Insert

PanK

Tags

6XHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSR47S Cm

Plasmid

#41892

Depositor

Howard Steinman

Type

Empty backbone

Use

Bacterial allelic exchange vector with sacb

Expression

Bacterial

Available Since

May 1, 2013

Availability

Academic Institutions and Nonprofits only
pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3-His

Plasmid

#11821

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

Plasmid

#160540

Purpose

Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Inserts

SARS-CoV-2 NSP7 (ORF1ab Synthetic)
SARS-CoV-2 NSP8 (ORF1ab Synthetic)
SARS-CoV-2 NSP12 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCas-Cas12m

Plasmid

#192274

Purpose

Encodes MmCas12m under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12

Use

CRISPR

Expression

Bacterial

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pKD182

Plasmid

#90952

Purpose

Expresses ThsS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

ThsS

Expression

Bacterial

Promoter

Ptac

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-hsFlt3L

Plasmid

#25074

Depositor

Maria Castro

Article

Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

Insert

human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)

Expression

Bacterial

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_Δpvc10

Plasmid

#198448

Purpose

Produces PVCs containing truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_Δpvc10

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINB-AMSH*

Plasmid

#66712

Purpose

Expresses activated AMSH in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

hbp (Stam2 Mouse)
AMSH (STAMBP Human)

Tags

His-3C

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_YWHAZ_14-3-3

Plasmid

#109808

Purpose

Protein expression and purification of YWHAZ_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAZ_14-3-3 (YWHAZ Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHC01

Plasmid

#106900

Purpose

Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

Depositor

Jonathan Silberg

Article

Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

Inserts

LasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein

Expression

Bacterial

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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rsZACRO/pRSETB

Plasmid

#236200

Purpose

Bacterial expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

Insert

rsZACRO

Expression

Bacterial

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a His6HuR

Plasmid

#135943

Purpose

Expresses HuR in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-B

Plasmid

#177812

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-MBP-His-SFP

Plasmid

#141141

Purpose

Expression of 4'-phosphopantetheinyl transferase gene

Depositor

New England Biolabs

Insert

4'-phosphopantetheinyl transferase

Tags

MBP

Expression

Bacterial

Available Since

May 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMD19T-psba1-TetR-PL22-dCas9-SpR

Plasmid

#73223

Purpose

Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PL22

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET His10 TEV LIC cloning vector (2B-T-10)

Plasmid

#78173

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSET-dCas9

Plasmid

#110183

Purpose

Expression of the dCas9 gene in Streptomyces

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Insert

dCas9

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

ermE*p

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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