32,870 results
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Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
Tags6xHistidine and HiBiTExpressionBacterialPromoterT7Available SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRExpressionBacterialAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a mScarlet
Plasmid#178543PurposeThe mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.DepositorInsertmScarlet
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FMRP_P451S
Plasmid#198693PurposeExpression of human FMRP protein in E. coliDepositorInsertFMR1 (FMR1 Human)
TagsMBPExpressionBacterialMutationchanged proline 451 to serinePromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb
Plasmid#198292PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobodyDepositorInsertPVCpnf_pvc13-antiMouseMHCIINb
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-UbE2D3
Plasmid#186805PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN-sacB
Plasmid#196249PurposePlasmid containing the sacB geneDepositorInsertattR2-sacB-attL3
ExpressionBacterialPromotersacB promoterAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miniGFP2
Plasmid#188897PurposeExpress 6xHis-Tag miniGFP2 in E.coliDepositorInsertminiGFP2
Tags6xHisExpressionBacterialPromoteraraBADAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
TagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBolux
Plasmid#188109PurposeEncodes a promoter-less Bacteroides-optimized lux cassetteDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106ExpressionBacterialAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcoFus
Plasmid#187833Purpose1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-hfYFP
Plasmid#186522PurposeHyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertHyperfolder YFP
Tags6xHisExpressionBacterialMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
ExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREDusk-MCS
Plasmid#188970PurposeFor red light-repressed bacterial expression of gene of interestDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFC0619
Plasmid#186690PurposeVcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargoDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
ExpressionBacterialPromoterPLacAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD3_mCherry
Plasmid#187385PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRTDepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL2310
Plasmid#187875PurposeEmpty pVS1-based INTEGRATE vector with loxP cargoDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only