30,753 results

1. pDSP31

   Plasmid

   #199373

   Purpose

   Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
   

   Expression

   Bacterial

   Mutation

   no mutations, except added subcellular localizati…

   Available Since

   April 25, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pQdC12a

   Plasmid

   #191356

   Purpose

   Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Inserts

   PGusA2-TetO2/1-dCas12a (LbCpf1)
   miniPthl-tetR
   

   Expression

   Bacterial

   Promoter

   PGus2A-TetO2/1 and miniPthl

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AsCpf1-2NLS

   Plasmid

   #102565

   Purpose

   bacterial expression of AsCpf1-2NLS

   Depositor

   Jennifer Doudna

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   AsCpf1
   

   Tags

   6xHis-MBP-TEV and HA-2xSV40NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScPPX1

   Plasmid

   #233713

   Purpose

   Bacterial expression of the yeast PPX1 gene

   Depositor

   Michael Downey

   Article

   Baijal et al bioRxiv 2025.02.12.637445

   Insert

   ScPpx1 (PPX1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   May 7, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDuet TDP-43 Q331K

   Plasmid

   #27465

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Coexpression

   Expression

   Bacterial

   Mutation

   Glutamine 331 mutated to Lysine (Q331K)

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30-GBFusion1

   Plasmid

   #133438

   Purpose

   Streptococcus GB1 domain in vector for bacterial expression.

   Depositor

   Gerhard Wagner

   Article

   Zhou et al J Biomol NMR. 2001 May . 20(1):11-4.

   Insert

   GB1 domain
   

   Expression

   Bacterial

   Mutation

   This fragment encodes only aa 1 to 56.

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-RhoA

   Plasmid

   #12202

   Depositor

   Jonathan Chernoff

   Article

   Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

   Insert

   RhoA (RHOA Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYHY3

   Plasmid

   #216109

   Purpose

   aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

   Depositor

   Shannon Sirk

   Article

   Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

   Insert

   NanoLuc
   

   Use

   Synthetic Biology; E. coli-bacteroides shuttle ve…

   Tags

   3xFLAG tag and BT3630 signal peptide

   Expression

   Bacterial

   Promoter

   P1TDP-A21

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDB179-TfCut2-S121P-D174S-D204P

   Plasmid

   #218695

   Purpose

   Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   TfCut2
   

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   S121P-D174S-D204P

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG024 (SIR2 + HerA)

    Plasmid

    #157902

    Purpose

    Expresses the SIR2 + HerA defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    SIR2 + HerA
    

    Expression

    Bacterial

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF-MCM4/6

    Plasmid

    #116951

    Purpose

    Expresses human MCM4 and human MCM6

    Depositor

    James Chong

    Article

    Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

    Inserts

    Human MCM6 (MCM6 Human)
    Human MCM4 (MCM4 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK647

    Plasmid

    #71701

    Purpose

    Produces E. coli thioredoxin reductase (EcTrxB)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDule2-para-aminoPhe

    Plasmid

    #85503

    Purpose

    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

    Insert

    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32T, E107T, D158P, I159L, L162A

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b_Q69L

    Plasmid

    #186272

    Purpose

    Expression vector of His-RanQ69L in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Tags

    His-tagged at N terminal

    Expression

    Bacterial

    Mutation

    Changed Q69 into L

    Promoter

    T7 promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 18 pCOLI_A_Trx

    Plasmid

    #160255

    Purpose

    N-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    6xHis and Thioredoxin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Tags

    Cerulean

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. cr3Dd

    Plasmid

    #184862

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, bet, exo
    

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ3538 eforRed-targeting sgRNA

    Plasmid

    #239455

    Purpose

    guide for eforRed

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting eforRed
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. ptac::ccdB

    Plasmid

    #15922

    Depositor

    Nina Papavasiliou

    Article

    Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

    Insert

    ccdB (ccdB E. coli)
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBP-P43

    Plasmid

    #112775

    Purpose

    Strong promoter activity during exponential and stationary phase

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    P43 promoter
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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