29,747 results

1. pET-CjCas9

   Plasmid

   #89754

   Purpose

   Expression of CjCas9 with His tag in E.coli

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   6XHis, HA, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-T7-NlaCas3-NLS-6xHis

   Plasmid

   #180214

   Purpose

   Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   NlaCas3c
   

   Use

   Tags

   6xHis and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAT2

   Plasmid

   #112583

   Purpose

   Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHis-LMW_FGF2

   Plasmid

   #157657

   Purpose

   Expresses FGF2 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changed Cystein 211,229 to serine, deleted amino …

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-SpCas9

   Plasmid

   #117051

   Purpose

   In vitro expression of S. pyogenes Cas9 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   S. pyogenes Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-PAmCherry-KanMX6

   Plasmid

   #87026

   Purpose

   Tag S. pombe genes in C terminal with PAmCherry

   Depositor

   Julien Berro

   Insert

   PAmCherry
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVRa42_4454

   Plasmid

   #49703

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF42_4454
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLS.405

   Plasmid

   #187203

   Purpose

   Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Inserts

   Retron Eco1 ncRNA (wildtype)
   Cas1 + Cas2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7/Lac

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. All_in_one_CRISPR/Cas9_LacZ

   Plasmid

   #74293

   Purpose

   "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

   Depositor

   Lynne Postovit

   Article

   Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

   Inserts

   LacZ-alpha
   Cas9
   mCherry
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Lac promoter, SV40, and T7 promoter

   Available since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS mFgfr1 (CT#92)

    Plasmid

    #14005

    Depositor

    Cliff Tabin

    Insert

    Fgfr1 (Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

11. p006-GFP-pBAD

    Plasmid

    #108315

    Purpose

    GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    GFP under control of arabinose-inducible promoter pBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRF-PsrA

    Plasmid

    #49368

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    PsrA repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pComb8

    Plasmid

    #63889

    Purpose

    For phage display of antibody libraries by fusion to modified geneVIII.

    Depositor

    Carlos Barbas

    Article

    Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

    Type

    Empty backbone

    Use

    Phage display

    Tags

    geneVIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C3-hYAP2

    Plasmid

    #17844

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAR357_SFP_pCDF-1b

    Plasmid

    #122851

    Purpose

    Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    Sfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-mhYFP

    Plasmid

    #186532

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    araB

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AK3L1

    Plasmid

    #25541

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AK3L1 (AK4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pXD68Kan2-AphA

    Plasmid

    #191248

    Purpose

    RSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b His-p97

    Plasmid

    #169020

    Purpose

    Expresses His-p97 in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge