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pBAD-Ag43

Plasmid

#107743

Purpose

Expresses Ag43 under the control of an L-arabinose transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Publication

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

Ag43 (flu )

Expression

Bacterial

Mutation

removed all pstI cut sites with silent mutations,…

Promoter

pBAD

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Publication

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC3_8

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUA66 theta GFP

Plasmid

#105607

Purpose

Expresses GFP with no promoter

Depositor

Pamela Silver

Publication

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Expression

Bacterial

Promoter

No Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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5KO9 (HMCES)

Plasmid

#101877

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HMCES (HMCES Human)

Tags

6x His (modified C-term tag is AENLYFQSHHHHHH)

Expression

Bacterial

Promoter

T7

Available since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pBAD-K-GECO1

Plasmid

#105864

Purpose

Bacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Publication

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Tags

6-His Tag

Expression

Bacterial

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBBR2k-GFPuv

Plasmid

#106383

Purpose

Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

tet

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-1-660

Plasmid

#104196

Purpose

Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Publication

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-660 (AR Human)

Tags

GST

Expression

Bacterial

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBAF

Plasmid

#104152

Purpose

Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

Depositor

Robert Craigie

Publication

Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

Insert

Barrier-to-autointegration factor (BAF) (BANF1 Human)

Tags

N-terminal His tag with thrombin cleavage site

Expression

Bacterial

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEB2-mRuby3

Plasmid

#104004

Purpose

Plasmid encoding mRuby3

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRuby3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEB2-mCherry2-L

Plasmid

#104003

Purpose

Plasmid encoding mCherry2-L

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pTECH-MmAcK3RS(IPYE)

Plasmid

#104071

Purpose

Expression plasmid encoding MmAcK3RS(IPYE) and pylT

Depositor

David Liu

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MmAcK3RS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-FGE

Plasmid

#102615

Purpose

Inducible expression of Formylglycine-generating enzyme

Depositor

Marc Wold

Publication

Bain et al Methods Enzymol. 2018;600:439-461. doi:

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Publication

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p11d-sctRPA

Plasmid

#102614

Purpose

Expression of S. cerevisiea RPA in E.coli

Depositor

Marc Wold

Publication

Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

Inserts

RPA1 (RFA1 Budding Yeast)
RPA2 (RFA2 Budding Yeast)
RPA3 (RFA3 Budding Yeast)

Expression

Bacterial

Mutation

RFA2 has intron deleted

Promoter

T7

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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SQ171fg/pRibo-T

Plasmid

#69343

Purpose

SQ171 fast growing strain supported by pRibo-T plasmid

Depositor

Michael Jewett

Publication

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

Ribo-T

Expression

Bacterial

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pC012 LwCas13a locus with nontargeting spacer

Plasmid

#91904

Purpose

LwCas13a locus into pACYC184 with nontargeting spacer

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a with non-targeting spacer

Use

CRISPR

Expression

Bacterial

Available since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pRM006

Plasmid

#98922

Purpose

Express Histag-SUMO-FtsZ for purification in E coli

Depositor

Jie Xiao

Publication

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsZ

Tags

Histag-SUMO

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Publication

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Publication

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBZcsx27

Plasmid

#89900

Purpose

Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

Depositor

Feng Zhang

Publication

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

csx27

Expression

Bacterial

Promoter

Lac

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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