-
Plasmid#174409PurposeC. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Para
Plasmid#218362PurposeEmpty backbone with araC-ParaBAD expression systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET3a aSyn murine
Plasmid#108865PurposeExpresses murine alpha synucleinDepositorInsertalpha synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attR4-pegRNA-attR3
Plasmid#213056PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attL4
Plasmid#213055PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR5
Plasmid#213053PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-His tagged nuclear Pif1
Plasmid#65047Purposeexpression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tagDepositorInsertYeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
UseTagshistagExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144B (27-236)
Plasmid#110753PurposeBacterial Expression of RNF144B RBR domainDepositorInsertRNF144B (RNF144B Human)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ec2Avs2
Plasmid#188790PurposeAvs2 from Escherichia coli NCTC10650DepositorInsertEc2Avs2
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJUMP45-2A(sfGFP)
Plasmid#126989PurposeLevel 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV LIC cloning vector (1O)
Plasmid#29658DepositorTypeEmpty backboneUseTags6xHis, Mocr, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK-GW
Plasmid#126929PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGM1
Plasmid#64949Purposesource of GmR FRT cassetteDepositorInsertFRT-GmR-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-SNAP25
Plasmid#168031PurposeExpresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cellsDepositorInserteCFP-SNAP25(A141-G206)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT12.G
Plasmid#201000PurposepLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApRDepositorInsertsuperfolder GFP
UseTagsExpressionBacterialMutationPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDSG594
Plasmid#214221Purposecompetition strain 10 integrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only