32,401 results
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Plasmid#138414PurposeProtein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tagDepositorInsertHimar-dCas9
Tags3xFLAG, 6xHis, and SV40 NLSExpressionBacterialPromoterT7Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-AMSH*
Plasmid#66712PurposeExpresses activated AMSH in E. coliDepositorAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mu-FLT3Lv1-pTRIP
Plasmid#163519PurposeUsed to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cellsDepositorAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR-Hyg
Plasmid#158708PurposeFor cloning and constitutive expression of crRNAs in mycobacteriumDepositorInsertcrRNA
UseCRISPR and Synthetic Biology; Mycobacteria expres…ExpressionBacterialPromoterHsp60Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Plasmid#140082PurposeEntry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoterDepositorInsertpT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
UseIn vitro transcription (mrna synthesis)ExpressionBacterialPromoterT7Available SinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-14-3-3 epsilon GST
Plasmid#13279DepositorAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLBH559_Tet-HisCas14a1Locus
Plasmid#112502PurposeExpresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14a1
UseCRISPRTags6xHis-TEVExpressionBacterialPromoterTetAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-NLS-6xHis (gbr2101)
Plasmid#181743Purposebacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterT7Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
TagsHis and MBPExpressionBacterialPromotertacAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoAp-GFPuv
Plasmid#139072PurposeLow phosphate inducible promoter phoAp for GFP expressionDepositorInsertphoAp-GFPuv
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRAF-MutS
Plasmid#86605PurposeE. coli MutS expression plasmidDepositorInsertE. coli MutS (D835R,R840E)
Tagshis-tag and ald-tagExpressionBacterialMutationD835R, R840EPromoterT7Available SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBluescript Sp5
Plasmid#16884DepositorInsertsp5 (sp5 Zebrafish)
ExpressionBacterialAvailable SinceJuly 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-His6-1_HBD
Plasmid#171869PurposeExpresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cellsDepositorInsertHybrid binding domain of Rnase H1
TagsGST- and His10-tagExpressionBacterialMutationWTAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS aP2 promoter (5.4kb) polyA
Plasmid#11424DepositorInsertaP2 promoter (Fabp4 Mouse)
ExpressionBacterialAvailable SinceApril 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PRDX6-Halo
Plasmid#175338PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR188
Plasmid#79543PurposeOutput plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS*-PBBa_J23106-geneIII
Plasmid#134352PurposePhagemidDepositorInsertGene III
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PprgH-GFPLVA
Plasmid#23340DepositorInsertGFP(LVA)
TagsGFP(LVA)ExpressionBacterialAvailable SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTnsABC
Plasmid#160031PurposeExpresses V. cholerae TnsABCDepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonExpressionBacterialMutationnoAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only