30,753 results

1. pET28a-SnoopTagJr-MBP

   Plasmid

   #105628

   Purpose

   Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

   Depositor

   Mark Howarth

   Article

   Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

   Insert

   SnoopTagJr-MBP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHLUM (version 2)

   Plasmid

   #64166

   Purpose

   harbours marker genes HIS3, LEU2, URA3 and MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. p406GAL1

   Plasmid

   #17437

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   GAL1 promoter is between SacI and XbaI restrictio…

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbS2c-RFP

   Plasmid

   #35329

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. KHK

   Plasmid

   #25532

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   KHK (KHK Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pLG002 (BREX type I)

   Plasmid

   #157880

   Purpose

   Expresses the BREX type I defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   BREX type I
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FKBP8

   Plasmid

   #25609

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   FKBP8 (FKBP8 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   FKBP-type PPIase Domain, residues 90-205

   Available Since

   Aug. 30, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. g5s pD42

   Plasmid

   #117452

   Purpose

   Expression of Cyth2

   Depositor

   Richard Kahn

   Insert

   Cyth2 (CYTH2 Human)
   

   Tags

   V5-His6

   Expression

   Bacterial

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-mCerulean-PA-Rac1

   Plasmid

   #22030

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Tags

   6X His and mCerulean

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3CEG

    Plasmid

    #60107

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    BIRC6:SDC136-F10:C41228 (BIRC6 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pPEPY-PF6-lacI

    Plasmid

    #85589

    Purpose

    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    lacI
    

    Expression

    Bacterial

    Promoter

    PF6

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMMB67EH-dddAI

    Plasmid

    #186559

    Purpose

    Broad host range regulated expression of the DddA immunity determinant (DddAI)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

    Plasmid

    #159539

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MBP-hnRNPA2_FL_WT

    Plasmid

    #98662

    Purpose

    Expresses MBP-tagged full length hnRNPA2

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid

    #111850

    Purpose

    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G12V (KRAS Human)
    

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Changed Glycine 12 to Valine

    Promoter

    T5

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM33_Nsp5_Mpro

    Plasmid

    #156475

    Purpose

    Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP728

    Plasmid

    #216374

    Purpose

    xNup358-Nb1t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup358-Nb1t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYN2_1

    Plasmid

    #184757

    Purpose

    Plasmid for genome editing by CRISPR/Cas9

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    Cas9
    sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
    

    Use

    Synthetic Biology

    Tags

    2xNLS

    Expression

    Bacterial and Yeast

    Promoter

    PGK1 and tRNA-Phe-HDV Ribozyme

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQLinkN

    Plasmid

    #13670

    Depositor

    Konrad Buessow

    Article

    Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

    Insert

    multi cloning site
    

    Use

    Co-expression

    Expression

    Bacterial

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV0279

    Plasmid

    #149688

    Purpose

    Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SUMO_Eu1-MBP
    

    Tags

    3xFLAG-tag and His14-tag

    Expression

    Bacterial

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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