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pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH45

Plasmid

#199767

Purpose

Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH45

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-M(BRCT)TdTwt

Plasmid

#207459

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

M(BRCT)TdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-FRT

Plasmid

#113398

Purpose

expresses gRNA for Cas9 FRT targetting

Depositor

Jan Michiels

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

exo, beta, gam, sgRNA-FRT

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMM656

Plasmid

#68887

Purpose

Rhamnose-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pET23a-H6-TEV-cSrc

Plasmid

#214233

Purpose

Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

Depositor

Neel Shah

Article

Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

Insert

cSrc Kinase domain (SRC Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2w/b

Plasmid

#111570

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mu-IFNg-pCIneo

Plasmid

#163517

Purpose

Expresses murine IFN gamma in mammalian cells

Depositor

Thomas Weber

Insert

murine-IFN gamma (Ifng Mouse)

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA_mTurquoise2

Plasmid

#108237

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter - mTurquoise2

Expression

Bacterial

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYHY1

Plasmid

#215926

Purpose

E. coli-Bacteroides shuttle vector.

Depositor

Shannon Sirk

Article

Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

Type

Empty backbone

Use

Synthetic Biology; E. coli-bacteroides shuttle ve…

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBBR4pemIK

Plasmid

#126928

Purpose

Broad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selection

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMP4639

Plasmid

#107771

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pME6010

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-msfGFP

Plasmid

#174492

Purpose

bacterial expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-mTET1

Plasmid

#81052

Purpose

bacterial expression of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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+36GFP-LPETG

Plasmid

#71754

Purpose

+36GFP-LPETG for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_(pos36GFP)_6XHis_LPETG

Tags

6XHis and Sortase (LPETG)

Expression

Bacterial

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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PPDK

Plasmid

#64037

Purpose

Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

Depositor

Vern Schramm

Article

Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

Insert

pyruvate phosphate dikinase

Tags

none

Expression

Bacterial

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only
pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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