30,753 results

1. pDSG323

   Plasmid

   #115594

   Purpose

   surface-displayed Null

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Expression

   Bacterial

   Available Since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis APEX2-eIF4A1

   Plasmid

   #129645

   Purpose

   Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

   Depositor

   Nicholas Ingolia

   Article

   Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

   Insert

   eukaryotic translation initiation factor 4A1 (EIF4A1 Human)
   

   Tags

   6xHis and APEX2

   Expression

   Bacterial

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE-80L MBP-SspB Nano

   Plasmid

   #60409

   Purpose

   E. coli expression of SspB Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   SspB nano
   

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   Y11K and A15E

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRE135

   Plasmid

   #118489

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. bdSUMO-pHlip-GFP-HiBit

   Plasmid

   #171016

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHlip-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET41b+_Ufd1-His

   Plasmid

   #117107

   Purpose

   Production of recombinant Ufd1-His in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Ufd1 (UFD1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pE2n

   Plasmid

   #17456

   Purpose

   modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCool-mRbx1-His-hCul1-CTP

   Plasmid

   #29519

   Depositor

   Ning Zheng

   Insert

   Rbx1 CUL1 (CUL1, Rbx1 Mouse, Human)
   

   Tags

   GST, His (before thrombin), and Thrombin Cut Site…

   Expression

   Bacterial

   Available Since

   May 26, 2011

   Availability

   Academic Institutions and Nonprofits only

9. GST-Delta23C11orf83

   Plasmid

   #65849

   Purpose

   bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion of the 23 first aa (TM)

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniTn7(Gm)PA1/04/03-eyfp-a

    Plasmid

    #111620

    Purpose

    eYFP Fluorescent tagging of bacteria at their Tn7-site

    Depositor

    Tim Tolker-Nielsen

    Article

    Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

    Insert

    PA1/04/03-eyfp
    

    Expression

    Bacterial

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFP.E222

    Plasmid

    #138248

    Purpose

    Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    ECF20.N.(VDA).M86.N2
    Terminator
    SspGyrB.C2.(SAK).ECF16.C
    Terminator
    rhaS
    ECF17.N.(NPC).NrdJ-1.N2
    Terminator
    M86.C2.(SDL).ECF20.C
    Terminator
    luxR
    Terminator
    ECF16.N.(AGG).SspGyrB.N2
    Terminator
    NrdJ-1.C2.(SEI).ECF17.C
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCP20T-morange

    Plasmid

    #78468

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    mOrange
    

    Expression

    Bacterial

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCold CL7-Cas12a

    Plasmid

    #124891

    Purpose

    obtaining full Cas12a RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas12a
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSETA_ionRD

    Plasmid

    #172924

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a-TEX264_wt-His6

    Plasmid

    #220214

    Purpose

    Bacterial expression of wild type TEX264, C-terminal His6 tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD_GFP11_T7LysH17A

    Plasmid

    #59591

    Purpose

    Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

    Depositor

    Richard Kingston

    Article

    Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

    Type

    Empty backbone

    Tags

    6xHis-tag and GFP11

    Expression

    Bacterial

    Promoter

    pARA

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Cis1-SH2

    Plasmid

    #46417

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Cis1 (SOCS1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 90-214

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-Streptavidin-K121R

    Plasmid

    #89880

    Purpose

    Streptavidin that has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K121R-Glutamate_Tag
    

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K121R

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. rbcLS-pMAL-2px

    Plasmid

    #41621

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    rbcL/rbcS
    

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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