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33,906 results

Showing: 4161 - 4180 of 33906 results

  2. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Publication
    Barre et al (unpublished)
    Insert
    EGFP
    Expression
    Bacterial and Yeast
    Promoter
    TET4 (contains four rtTA binding sites)
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  3. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Publication
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    177334-plasmid-map-sequence-id-348602

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  4. pRelA*

    Plasmid
    #175590
    Purpose
    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Publication
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Tags
    Fused with a flexible GS linker to mVenus
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175590-plasmid-map-sequence-id-348362

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  5. pMesh1*

    Plasmid
    #175591
    Purpose
    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Publication
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1 (Mesh1 Fly)
    Tags
    Fused with a flexible GS linker to cerulean
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175591-plasmid-map-sequence-id-348365

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  13. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Publication
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  14. pSUB11

    Plasmid
    #169699
    Purpose
    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Publication
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    3xFLAG tag
    Expression
    Bacterial
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169699-plasmid-map-sequence-id-336760

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  15. pSCrhaB2plus

    Plasmid
    #164226
    Purpose
    Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.
    Depositor
    Silvia Cardona
    Publication
    Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    Rhamnose-inducible
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    164226-plasmid-map-sequence-id-325696

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  18. pM1s3AsR_TS-C7

    Plasmid
    #137945
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression
    Depositor
    Neel Joshi
    Publication
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    137945-plasmid-map-sequence-id-331373

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  20. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

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Showing: 4161 - 4180 of 33906 results