29,754 results

1. pET11d-RAN

   Plasmid

   #108919

   Purpose

   Expresses human RAN

   Depositor

   Larry Gerace

   Article

   Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

   Insert

   RAN (RAN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM2 hTSP-1

   Plasmid

   #12993

   Depositor

   Jack Lawler

   Insert

   TSP-1 (THBS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBHM2641

   Plasmid

   #229525

   Purpose

   Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   CRISPR

   Tags

   t2a-mNeonGreen-AID*

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   TEF1

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBHM2642

   Plasmid

   #229526

   Purpose

   Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   Tags

   t2a-mNeonGreen-mIAA7_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBHM2644

   Plasmid

   #229528

   Purpose

   Carries AID* tag, empty sgRNA, and NATR marker

   Depositor

   Hiten Madhani

   Insert

   AID* - empty sgRNA - NATR
   

   Use

   Tags

   2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBHM2645

   Plasmid

   #229529

   Purpose

   Carries mIAA7_opt tag, empty sgRNA, and NATR marker

   Depositor

   Hiten Madhani

   Insert

   mIAA7_opt - empty sgRNA - NATR
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBHM2646

   Plasmid

   #229530

   Purpose

   Carries mAID_opt tag, empty sgRNA, and NATR marker

   Depositor

   Hiten Madhani

   Insert

   mAID_opt - empty sgRNA - NATR
   

   Use

   Tags

   2xFLAG

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBHM2647

   Plasmid

   #229989

   Purpose

   Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   Tags

   t2a-mNeonGreen-mIAA7_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBHM2648

   Plasmid

   #229990

   Purpose

   Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Use

   Tags

   t2a-mNeonGreen-mIAA7_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBHM2649

    Plasmid

    #229991

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBHM2650

    Plasmid

    #229992

    Purpose

    Carries AID* tag, empty sgRNA, and HYGR marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - HYGR
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBHM2651

    Plasmid

    #229993

    Purpose

    Carries AID* tag, empty sgRNA, and NEOR marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - NEOR
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBHM2652

    Plasmid

    #229994

    Purpose

    Carries AID* tag, empty sgRNA, and amdS marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - amdS
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMRS-XylS-dCas9

    Plasmid

    #220196

    Purpose

    mini-Tn7 dCas9 expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    XylS/pM

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpyB

    Plasmid

    #220186

    Purpose

    sgRNA expression vector - pBG35 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBG35

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pET-AqpZ-ALFA

    Plasmid

    #212310

    Purpose

    Expression of AqpZ with ALFA tag

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    Aquaporin Z
    

    Use

    Tags

    ALFA tag and Strep-Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET19b+H3

    Plasmid

    #157807

    Purpose

    Expression of cysteine-free human histone H3 (c111A)

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C111A

    Promoter

    T7

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS hTSP-5

    Plasmid

    #13002

    Depositor

    Jack Lawler

    Article

    Newton et al Genomics. 1994 Dec . 24(3):435-9.

    Insert

    COMP (COMP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid

    #196185

    Purpose

    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Inserts

    GSK3B (GSK3B Human)
    λPPase
    

    Use

    Tags

    GST, HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge