30,756 results
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pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-Cre-HiBiT
Plasmid#198318PurposeLoads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains PnfDepositorInsertPdp1_NTD-Cre-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-gst-xr
Plasmid#214761PurposeXylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coliDepositorInsertglutathione S-transferases fused with xylose reductase
TagsGlutathione S-transferasesExpressionBacterialPromoterT7Available SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-FimH-9xHis
Plasmid#97305PurposeE.coli UTI89 FimH signal peptide and lectin domainDepositorInsertType 1 fimbiral adhesin FimH
Tags9xHisExpressionBacterialAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG166
Plasmid#124366PurposeGfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilisDepositorAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST HP1 gamma
Plasmid#24076DepositorAvailable SinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-neo
Plasmid#71320PurposeDonor plasmid - kanamycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
WT-eGFP
Plasmid#215168PurposeEncodes the enhanced green fluorescent proteinDepositorInsertWT-eGFP
TagsHis-tagExpressionBacterialPromoterT7 lacAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PRKACB_Pkinase
Plasmid#110024PurposeProtein expression and purification of PRKACB_PkinaseDepositorInsertPRKACB_Pkinase (PRKACB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pgRNA
Plasmid#215560PurposeConstitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanRDepositorInsertgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119Available SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH-GFPminimizer
Plasmid#202488PurposeExpresses the nanobody GFP minimizer with T7 inductionDepositorInsertnanobody GFP minimizer
Tags6XHisExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_Q331K
Plasmid#98673PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with Q331KDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUA66 PrpsL GFP
Plasmid#105606PurposeExpresses GFP under PrpsLDepositorInsertGFP
ExpressionBacterialPromoterPrpsLAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas12f1
Plasmid#171182PurposeConstitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1DepositorInsertCas12f1
UseCRISPRExpressionBacterialPromotercas9 promoter from Streptococcus pyogenesAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGAM5
Plasmid#38976PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENV8(WT)-GFPuv
Plasmid#70127PurposeCobalamin riboswitch reporter vector for use in E. coliDepositorInsertwild type environmental cobalamin riboswitch sequence (env8)
UseSynthetic BiologyTagsNoneExpressionBacterialPromotertacAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
poRibo-T2
Plasmid#69347PurposeRibo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutationDepositorInsertoRibo-T
ExpressionBacterialAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
RBD#4L
Plasmid#193021PurposehnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13aDepositorInsertCas13a
TagshnRNP C RRMExpressionBacterialAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only
