33,128 results

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pG1AK

Plasmid

#71736

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSLC-237

Plasmid

#73191

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

ParaB-relE

Expression

Bacterial

Promoter

ParaB

Available Since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-USP11

Plasmid

#78849

Purpose

To purify GST-tagged recombinant USP11 protein

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

Insert

USP11 (USP11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-RIPK3

Plasmid

#78827

Purpose

To purify GST-tagged recombinant RIPK3 protein

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAN3945

Plasmid

#74699

Purpose

Circuit plasmid: 0x41

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x41

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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AcpP pet29a C-terminal His Tag

Plasmid

#75016

Purpose

Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

AcpP (acpP E. coli)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pTrcECT

Plasmid

#75477

Purpose

Inducible expression of ectABC in E. coli

Depositor

Xixian Xie

Article

Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

Insert

ectABC

Expression

Bacterial

Promoter

trc

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-MAP

Plasmid

#66999

Purpose

Expresses malE-MAP in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-MAP

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRL692

Plasmid

#70284

Purpose

Tn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942

Depositor

Peter Wolk

Article

Koksharova et al J Bacteriol. 2002 Oct;184(19):5524-8.

Insert

Transposon Tn5-692

Expression

Bacterial

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pENV8(WT)-GFPuv

Plasmid

#70127

Purpose

Cobalamin riboswitch reporter vector for use in E. coli

Depositor

Robert Batey

Article

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

wild type environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSNAP-CAPZ

Plasmid

#69948

Purpose

Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

Depositor

Jeff Gelles

Article

Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

Inserts

CapZ beta 1 (CAPZB Chicken)
CapZ alpha 1 (CAPZA1 Chicken)

Tags

His 6 and SNAP

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT593

Plasmid

#68909

Purpose

NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pB3.P11

Plasmid

#69577

Purpose

OT-I TCR beta chain cDNA in pCRScript

Depositor

Francis R. Carbone

Article

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR beta cDNA clone B3 (Tcrb Mouse)

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBSKrnaSeph

Plasmid

#67506

Purpose

Express the tRNALys scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pLJSRSF3

Plasmid

#64692

Purpose

His-GST-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, GST, HisTag, NpuDnaE-N102, and Smt3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBW400hrpL-gfp

Plasmid

#61437

Purpose

pBW400hrpL-gfp

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

hrpL-rbs30-gfp-ter

Expression

Bacterial

Promoter

HrpL

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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