32,669 results
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Plasmid#132661PurposeRSF1010-based broad host-range plasmid with additional RK2 bom site and pUC originDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCri-1b
Plasmid#61327PurposeProtein expression.DepositorInsertGreen fluorescent protein
TagsHis-tag and MBP-tagExpressionBacterialAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTnsABC
Plasmid#160031PurposeExpresses V. cholerae TnsABCDepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonExpressionBacterialMutationnoAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL2-pCMV7.1
Plasmid#155020PurposeN-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vectorDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOP3MT
Plasmid#112601PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusionsDepositorTypeEmpty backboneTagsAvi - 8His-MBPExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHJL2
Plasmid#199377PurposeEncodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6f::SL2::mScarlet-I
ExpressionBacterialMutationno mutationsAvailable SinceApril 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
L4440_BioBrick-SCR-sgRNA-B
Plasmid#177812PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-SpCas9mSA-His
Plasmid#182951PurposeExpress SpCas9mSA in bacteriaDepositorInsertNLS-SpCas9mSA-His
UseCRISPRExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_RSV
Plasmid#175958PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]DepositorInsertsP gene
M gene
ExpressionBacterialPromoterT7Available SinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialPromoterT7Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Tn5
Plasmid#127917PurposeProkaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.DepositorInsertTn5
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7Available SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-TRC8 513-664
Plasmid#185352PurposeRING domain-containing GST fusion protein for ubiquitination assays.DepositorAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC018 - LshCas13a from Leptotrichia shahii
Plasmid#91908PurposeExpresses LshCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLshCas13a and guide expression cassette
ExpressionBacterialAvailable SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NM23-H1
Plasmid#157987PurposeExpresses NM23-H1 in E.coli cellDepositorInsertNM23-H1 (NME1 Human)
ExpressionBacterialAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-eGFP-dspB(E184Q)-6xHis
Plasmid#175783PurposePlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tag and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-BE3∆UGI
Plasmid#96943PurposeThe plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).DepositorInsertBE3∆UGI
TagsHis6 tagExpressionBacterialPromoterT7Available SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top2A-5X Gly-Venus-T2A-Hygro
Plasmid#211115PurposeFor tagging the C-term of Top2A with VenusDepositorInsertsUseCRISPRExpressionBacterial and MammalianPromotern/aAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 fosA
Plasmid#112889PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertfosA
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only