33,906 results

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pSR78

Plasmid

#90210

Purpose

Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA

Depositor

Jeffrey Tabor

Publication

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

CcaS
Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pRAU171

Plasmid

#86835

Purpose

bacterial expression of anti-CRISPR protein AcrIIA2

Depositor

Joseph Bondy-Denomy

Publication

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA2

Expression

Bacterial

Promoter

araBAD

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pJYS2.1-crtYf

Plasmid

#89077

Purpose

Constitutive transcription of crRNA of crtYF. CRISPR of C. glutamicum.

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYF

Use

CRISPR

Expression

Bacterial

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(C163S)

Plasmid

#90088

Purpose

Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

Depositor

Clay Clark

Publication

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

C163S

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET-15b-hAR-663-919

Plasmid

#89083

Purpose

bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR ligand binding domain (AR Human)

Tags

His

Expression

Bacterial

Mutation

aa 663-919

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLZ12Km2-P23R:TA:fflucRT

Plasmid

#88901

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Publication

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + Red-shifted firefly luciferase

Expression

Bacterial

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-NLS-cTF

Plasmid

#87746

Purpose

IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

Depositor

Wayne Patrick

Publication

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and NLS linker - cTF (chimeric transcriptio…

Expression

Bacterial

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pA5c-tesA-vhb50-8fadR

Plasmid

#86601

Purpose

expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

Depositor

Yinjie Tang

Publication

Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

Inserts

tesA
Vhb50
fadR

Expression

Bacterial

Promoter

LacUV5 and pBAD

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cIopt-J23106-geneIII

Plasmid

#80852

Purpose

Phagemid (encodes M13 gene III and lambda cIopt).

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
Lambda cIopt

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJPC12-ΔM13-mCherry-PR/PRM-GFP

Plasmid

#80859

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

Lambda Pr and Lambda Prm

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p2CT-His-MBP-Lbu_C2c2_R1079A

Plasmid

#83494

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1079A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1079A

Promoter

T7

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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DYR1A_HUMAN_D0

Plasmid

#79690

Purpose

This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DYRK1A (DYRK1A Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRAM18dRGA[MCS]

Plasmid

#84683

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Publication

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET30-2-GPD1

Plasmid

#83911

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI-J23106-geneIII

Plasmid

#80843

Purpose

Phagemid (encodes M13 gene III and lambda cIopt).

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
Lambda cI

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

Plasmid

#81185

Purpose

Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

Depositor

Jennifer Doudna

Publication

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Inserts

Cas5c
Cas8c
Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-SdeA

Plasmid

#80837

Purpose

Bacterial expression of SdeA

Depositor

Zhao-Qing Luo

Publication

Qiu et al Nature. 2016 May 5;533(7601):120-4. doi:

Insert

SdeA

Tags

6xHis

Expression

Bacterial

Promoter

T5

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTriEx-mCherry-LOVSD

Plasmid

#81033

Purpose

Mammalian expression of the super-dark state of LOV2

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LOVSD

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A, L514K, G528A, L531E, N538E

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHis17_ParM _1

Plasmid

#78201

Purpose

Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Publication

Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

Insert

parM (K33A I27C T174A T175N C287A)

Tags

His6

Expression

Bacterial

Mutation

K33A I27C T174A T175N C287A

Promoter

unknown

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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