31,597 results
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Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
Tagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pSB233
Plasmid#164128PurposePP7-CFP and PhlF-RFP expression plasmidDepositorInsertPP7-CFP
ExpressionBacterialPromoterPtetAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFP.R268
Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJP_Osc05
Plasmid#229040PurposeLight inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.DepositorInsertpBAD promoter
ExpressionBacterialAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
ExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable SinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9_CL
Plasmid#166245Purposeregulated dCas9 generatorDepositorInsertP112-sgRNA-term-P104-RBS1-dCas9-B15
UseSynthetic BiologyExpressionBacterialPromoterEc-TTL-P112 and Ec-TTL-P104Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_47
Plasmid#109245PurposepSC101 var5 BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pC28-LIC-sfGFP-His
Plasmid#165479PurposepET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tagDepositorTypeEmpty backboneTagsTEV-GFP-6xHisExpressionBacterialAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTCF5 HLA-A*01:01
Plasmid#180448PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*01:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT004-LaG30VB
Plasmid#163384Purpose2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN systemDepositorInsertLaG30-VEEGFRtm-BFP (LaG30VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b - PA
Plasmid#84863Purposebacterial expression of Bacillus anthracis protective antigenDepositorInsertPA
TagspelB signal sequenceExpressionBacterialPromoterT7Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY002 (pFnCpf1_min)
Plasmid#69975PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes nd artificial promoter
UseCRISPRExpressionBacterialAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iGluSnFR4f
Plasmid#234444PurposeBacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)DepositorInsertiGluSnFR4f
Tags6xHis-Xpress epiExpressionBacterialPromoterT7Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlk_N + pSiMPlk_C
Plasmid#134310PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertkanR_gp41-1 N-intein + kanR_gp41-1 C-intein
ExpressionBacterialAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOGG202
Plasmid#115503PurposeClone of a level 1 unit which encodes IPTG-inducible sfGFPDepositorInsertpL1M-F1-plac pOGG031, sfGFP pOGG037, T-pharma pOGG003
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -