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pETM6-G6-vioABE-4A6-vioC

Plasmid

#73439

Purpose

Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

Depositor

Mattheos Koffas

Publication

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE-P4A6-vioC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6 and P4A6 (orthogonal T7-lac variants)

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCORE-Kp53

Plasmid

#72241

Purpose

Contains the CORE cassette with KanMX4 and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
kanMX4

Expression

Bacterial

Mutation

V122A mutation

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-MAP

Plasmid

#66999

Purpose

Expresses malE-MAP in E. coli

Depositor

Ashutosh Chilkoti

Publication

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-MAP

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRL692

Plasmid

#70284

Purpose

Tn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942

Depositor

Peter Wolk

Publication

Koksharova et al J Bacteriol. 2002 Oct;184(19):5524-8.

Insert

Transposon Tn5-692

Expression

Bacterial

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pENV8(WT)-GFPuv

Plasmid

#70127

Purpose

Cobalamin riboswitch reporter vector for use in E. coli

Depositor

Robert Batey

Publication

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

wild type environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSNAP-CAPZ

Plasmid

#69948

Purpose

Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

Depositor

Jeff Gelles

Publication

Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

Inserts

CapZ beta 1 (CAPZB Chicken)
CapZ alpha 1 (CAPZA1 Chicken)

Tags

His 6 and SNAP

Expression

Bacterial

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Publication

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAT593

Plasmid

#68909

Purpose

NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pB3.P11

Plasmid

#69577

Purpose

OT-I TCR beta chain cDNA in pCRScript

Depositor

Francis R. Carbone

Publication

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR beta cDNA clone B3 (Tcrb Mouse)

Expression

Bacterial

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBSKrnaSeph

Plasmid

#67506

Purpose

Express the tRNALys scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Publication

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Tags

6xHis

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pBW400hrpL-gfp

Plasmid

#61437

Purpose

pBW400hrpL-gfp

Depositor

Martin Buck, Baojun Wang

Publication

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

hrpL-rbs30-gfp-ter

Expression

Bacterial

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTXB3-Flag PLK1

Plasmid

#68271

Purpose

bacterial expression of Plk1

Depositor

Stefano Ferrari

Insert

PLK1 (PLK1 Human)

Tags

Flag and Mxe intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSMART-GFPuv

Plasmid

#65822

Purpose

GFPuv Expression, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Publication

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

gfpuv

Expression

Bacterial

Promoter

E . coli insulated waaH gene promoter, low phosp…

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pPTDH

Plasmid

#66183

Purpose

Expresses phosphite dehydrogenase in E. coli

Depositor

Teruyuki Nagamune

Publication

Watanabe et al ChemCatChem 2013 Dec;5(12):3835-3840

Insert

Phosphite Dehydrogenase

Tags

His6 tag

Expression

Bacterial

Mutation

E175A, A176R

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK068

Plasmid

#65175

Purpose

Encodes ConR2 as a Type 5 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConR2

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-HxB.S

Plasmid

#65415

Purpose

contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

Depositor

Harold Erickson

Publication

Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

Insert

tenascin C small splice variant (TNC Human)

Expression

Bacterial

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET-XS-VWF

Plasmid

#64847

Purpose

Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

Depositor

Sriram Neelamegham

Publication

Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

Insert

Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Synthetic, Human)

Tags

7X His, Cerulean, and Venus

Expression

Bacterial

Promoter

T7

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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