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R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

Plasmid

#159700

Purpose

E. coli protein expression

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pIntegrase_4

Plasmid

#60576

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_4

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Article

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSLS.400

Plasmid

#187201

Purpose

Expresses retron Eco4 RT

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco4 Reverse Transcriptase

Use

Synthetic Biology

Expression

Bacterial

Promoter

mphR

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pET-28GG

Plasmid

#104655

Purpose

Level 2 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET19b+H3

Plasmid

#157807

Purpose

Expression of cysteine-free human histone H3 (c111A)

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H3

Expression

Bacterial

Mutation

C111A

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6*)-mCherry

Plasmid

#168048

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Article

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101* and PBAD

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET mOrange LIC cloning vector (u-mOrange)

Plasmid

#29770

Depositor

Scott Gradia

Type

Empty backbone

Tags

mOrange

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pAKlux2.1

Plasmid

#14081

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Expression

Bacterial

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

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pRF-SrpR

Plasmid

#49372

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

SrpR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pQcre1

Plasmid

#135659

Purpose

Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Cre recombinase

Use

Cre/Lox

Expression

Bacterial

Promoter

ppag promoter

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-H12TEV

Plasmid

#208635

Purpose

for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

Depositor

Quanfu Ma

Type

Empty backbone

Tags

6xhis

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pHC01

Plasmid

#106900

Purpose

Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

Depositor

Jonathan Silberg

Article

Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

Inserts

LasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein

Expression

Bacterial

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pFP.E222

Plasmid

#138248

Purpose

Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDR111_Bgal no NLS

Plasmid

#188397

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

Phyper spank

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHCL151

Plasmid

#134455

Purpose

TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

linker-mScarlet-I for C-terminal fusion

Expression

Bacterial

Promoter

plac

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-StayGold

Plasmid

#211360

Purpose

Bacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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