34,213 results
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Plasmid#165959PurposeExpression of soluble E. coli Tus proteinDepositorInsertTus
TagsHis-NusA-TevExpressionBacterialPromoterT7Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT204
Plasmid#134881PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-ALFA-His
Plasmid#171569PurposeBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)DepositorPublicationInsertNbVHH05-ALFA-His (UBE2J1 Human)
TagsALFA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-HA-His
Plasmid#171567PurposeBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)DepositorPublicationInsertNbVHH05-HA-His (UBE2J1 Human)
TagsHA-tag/His-tag and PelB signal peptideExpressionBacterialAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ4
Plasmid#167133PurposeA kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterKanamycin promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#154138PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorPublicationInsertfucO, aldA
ExpressionBacterialPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b-17X-PTDH
Plasmid#166786PurposePhosphite dehydrogenase for NAD(P)H regenerationDepositorInsertPhosphite dehydroenase
TagsHis6ExpressionBacterialMutationA196R, T201S, A328T, E352N, and C356D- please see…PromoterT7Available sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF-CjPglB-LpxE
Plasmid#128389PurposeExpression of C. jejuni PglB and F. tularensis LpxE in E. coliDepositorInsertsPglB oligosaccharylktransferase
LpxE phosphatase
TagsFlag tagExpressionBacterialPromoterPtac and pBADAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-EcMscL-TEV-mEGFP
Plasmid#165097PurposeExpresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.DepositorInsertE. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
UseSynthetic BiologyTagsmonomeric Enhanced Green Fluorescent ProteinExpressionBacterialPromoterT7Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluA-NP
Plasmid#165092Purposeprotein expression in E. coliDepositorInsertInfluenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
ExpressionBacterialAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCB’
Plasmid#162703PurposepCB reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertpHnCB10 derived carboxysome operon, prk
ExpressionBacterialMutation5513T>G (tetR E37A); 7758G>T (second tet op…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM1s3TsR
Plasmid#137922PurposeE. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-Cas9-recX
Plasmid#158715PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recXDepositorInsertcas9
UseCRISPRExpressionBacterialPromoterTetOAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNG1256
Plasmid#149710PurposeExpresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complexDepositorAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG023 (dsr2)
Plasmid#157901PurposeExpresses the dsr2 defense systemDepositorInsertdsr2 (SIR2)
ExpressionBacterialAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
ExpressionBacterialAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG032 (ppl)
Plasmid#157910PurposeExpresses the ppl defense systemDepositorInsertppl (PHP-ATPase)
ExpressionBacterialAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dual opposing T7 promoters reporter plasmid
Plasmid#156457PurposeA mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.DepositorInsertNeoR/KanR
ExpressionBacterialMutationATG start codon mutated to ACGPromoterEm7Available sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only