30,535 results

1. pREDusk-MCS

   Plasmid

   #188970

   Purpose

   For red light-repressed bacterial expression of gene of interest

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFCyR2

   Plasmid

   #167513

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymRAM-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCymRC

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRECMYC-Ebu1

   Plasmid

   #240713

   Purpose

   pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

   Depositor

   Seth Shipman

   Insert

   Ebu1 RT, Eco1 ncRNA, MspRecT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available Since

   Aug. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAK184.tetR.PT5-3.dCas9

   Plasmid

   #232482

   Purpose

   optimized ahTet inducible dCas9 for insertion into C. difficile genome

   Depositor

   James Collins

   Article

   Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

   Insert

   Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-PT5-3

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJC.15A.sgRNA.TA

   Plasmid

   #232483

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

   Depositor

   James Collins

   Article

   Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

   Inserts

   Toxin-antitoxin System from CD630
   medium-copy-number p15A origin of replication
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3 constitutive promoter and native promoter

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-LIC-N8C_Fab2b

   Plasmid

   #203692

   Purpose

   Expresses N8C_Fab2b

   Depositor

   Brenda Schulman

   Article

   Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

   Insert

   N8C_Fab2b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pE5770

   Plasmid

   #170777

   Purpose

   expression of hybrid proteins in E. coli

   Depositor

   Anthony Schryvers

   Article

   Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

   Insert

   Kan resistance (kanR )
   

   Use

   Tags

   polyhisbioMbpTEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZ8-T_dCas9

   Plasmid

   #74062

   Purpose

   pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   dcas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDule-pCNF

   Plasmid

   #85494

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWP78-PVCpnf_pvc13-Ad5RGDPK7

    Plasmid

    #198291

    Purpose

    Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions)

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-Ad5RGDPK7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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