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Showing: 441 - 460 of 29646 results

  4. pNeae2

    Plasmid
    #168300
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:
    Insert
    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    Use
    Tags
    6xHis, E-tag, and myc-tag
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168300-plasmid-map-sequence-id-334450

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  8. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Anat Herskovits
    Article
    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)
    Insert
    pheS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A309G
    Promoter
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98783-plasmid-map-sequence-id-189220

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  9. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    IPTG
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167546-plasmid-map-sequence-id-332204

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  10. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  11. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Kenneth Peterson
    Article
    Kovach et al Gene. 1995 Dec 1;166(1):175-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Available since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    85168-plasmid-map-sequence-id-372970

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  12. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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Showing: 441 - 460 of 29646 results