31,561 results
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Plasmid#232322PurposepZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.DepositorInsertmCherry
ExpressionBacterialPromoterrpsJAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKD236-4b
Plasmid#90956PurposeExpresses ThsS constitutivelyDepositorInsertThsS
ExpressionBacterialPromoterBba_J23104Available SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD237-3a-2
Plasmid#90957PurposeExpresses ThsR and mCherry constitutively and sfGFP under the PphsA promoterDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23100, Bba_J23105, and PphsAAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-pBAD
Plasmid#54844PurposeLocalization: Protein Expression Vector , Excitation: 434, Emission: 474DepositorTypeEmpty backboneTags6x His, T7 tag, and TEV siteExpressionBacterialPromoteraraBADAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMCSG53-DsbC
Plasmid#186623PurposeE. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.DepositorTypeEmpty backboneTagsDsbC-6His-TEVExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
TagsHis tagExpressionBacterialPromoterT7 PromoterAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPSU10-30-50-100
Plasmid#173838PurposeCoexpresses 10, 30, 50 and 100 kD Penn State ladder proteinsDepositorInsertSTRHSTPAB
ExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN251
Plasmid#188471PurposeLentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)DepositorInsertenCas12a-HF (gift from Dr. Keith Joung)
UseLentiviralExpressionBacterial and MammalianAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7II-2N3Rtau
Plasmid#249551PurposeRecombinant protein synthesis of 2N3R isoform of human TauDepositorInsertTau (2N3R) (MAPT Human)
ExpressionBacterialAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialAvailable SinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
ExpressionBacterialPromoterN/AAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSU20-40-60-80
Plasmid#173839PurposeCoexpresses 20, 40, 60 and 80 kD Penn State ladder proteinsDepositorInsertSTRHSTPABPACCBPv3
ExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTP1183
Plasmid#104163PurposeBacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)DepositorInsertAnti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
TagsHisExpressionBacterialMutationWt geneAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET GFP LIC cloning vector (2GFP-T)
Plasmid#29716DepositorTypeEmpty backboneTags6xHis, SiriusGFP, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only