30,803 results

1. SUMO-PNGaseF

   Plasmid

   #228498

   Purpose

   10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   ngl
   

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFN18A-HaloTag-Biotin

   Plasmid

   #206039

   Purpose

   HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Insert

   Talin R3-IVVI (Tln1 Mouse)
   

   Tags

   10 x His-AviTag and Halo-Tag

   Expression

   Bacterial

   Mutation

   Threonine 809 to Isoleucine, Threonine 833 to Val…

   Promoter

   T7

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PARP15-c020

   Plasmid

   #173101

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP15 (PARP15 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N78-S267, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSNRW-Z5-Nluc

   Plasmid

   #174487

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z5-Nluc
   

   Tags

   6x Histidine tag (N terminal on insert)

   Expression

   Bacterial

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24b-BG1.3

   Plasmid

   #199437

   Purpose

   expresses BAmGreen1.3 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen1.3
   

   Tags

   his tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRM053 His-TEV-Cas9 triNLS 0C

   Plasmid

   #196244

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

   Plasmid

   #159579

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

   Depositor

   Dominic Esposito

   Article

   Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

   Insert

   His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-CPDSalI

   Plasmid

   #38252

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. HeLa full-length MLCK-GFP

   Plasmid

   #46316

   Depositor

   Anne Bresnick

   Article

   Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

   Insert

   HeLa long myosin light chain kinase (MYLK Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Cys-ELP(40nm)-LPETGG

    Plasmid

    #90475

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    Cys-ELP(40nm)-LPETGG
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETZZtevPP7CP(C68A,C73A)

    Plasmid

    #27548

    Depositor

    Kathleen Collins

    Article

    Hogg et al RNA. 2007 Jun . 13(6):868-80.

    Insert

    Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
    

    Tags

    TEV protease cleavage site, six histidine tag, an…

    Expression

    Bacterial

    Mutation

    changed two cysteine to alanine (C68A,C73A) to in…

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2CT-His-MBP-Lse_C2c2_WT

    Plasmid

    #83486

    Purpose

    Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lse_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLBH559_Tet-HisCas14a1Locus

    Plasmid

    #112502

    Purpose

    Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11a-hRanGAP1

    Plasmid

    #50941

    Purpose

    expression of full length untagged human RanGAP1 in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanGTPase activating protein 1 (RANGAP1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-eLACCO1.1

    Plasmid

    #167944

    Purpose

    Fluorescent biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTHSSd_34

    Plasmid

    #59960

    Purpose

    PTac GFP expression

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

    Insert

    sfGFP
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    PTac

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAL-hCul1-NTD

    Plasmid

    #29518

    Depositor

    Ning Zheng

    Insert

    CUL1 (CUL1 Human)
    

    Tags

    Thrombin Cleavage Site

    Expression

    Bacterial

    Mutation

    N-terminal 415 aa

    Available Since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pT/SVNeo

    Plasmid

    #24550

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    pT/SVNeo
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

19. GFP E17TAG

    Plasmid

    #68295

    Purpose

    E17TAG GFP reporter

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPS0381

    Plasmid

    #133231

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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