29,771 results

1. pET28a_T5-ARG1

   Plasmid

   #106476

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Tn7-144

   Plasmid

   #118560

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mOrange2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Plasmid

   #166421

   Purpose

   Express pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Naegleria fowleri PRL
   

   Use

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lac1

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHis(TEV)-Drp1(K38A)-StrepII

   Plasmid

   #174423

   Purpose

   Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K38A

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pScaf-5544.4

   Plasmid

   #111524

   Purpose

   pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)

   Depositor

   Shawn Douglas

   Article

   Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

   Insert

   pScaf-KH'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXZ13lacZalpha

   Plasmid

   #160229

   Purpose

   places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

   Depositor

   Ian Duncan

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAJM.690

   Plasmid

   #108533

   Purpose

   PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PcaUAM + P3B5B-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   PcaU: K14N A165T N201H

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICSFAST-LY-MT-P2A

   Plasmid

   #214897

   Purpose

   Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-LY-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GST-FUS-WT

   Plasmid

   #44978

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS (Full length) (FUS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

10. Parp14-c011

    Plasmid

    #173098

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K791-F978

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMTEZ-TeTxLC

    Plasmid

    #32640

    Depositor

    Joseph Gogos, C. Ron Yu, Richard Axel

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Tetanus toxin light chain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSETA_crGE

    Plasmid

    #172927

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

    Depositor

    Arnold Boersma

    Article

    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

    Insert

    CrowdingSensorGE
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCVL.SSA TLR (Sce)

    Plasmid

    #45576

    Purpose

    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185bp, truncated 25 …

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. RhoA

    Plasmid

    #55699

    Purpose

    expression of mouse RhoA

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-PFO

    Plasmid

    #92109

    Purpose

    Recombinant expression in E.Coli

    Depositor

    Feng Shao

    Article

    Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

    Insert

    PFO
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Delta27aa

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PIA900

    Plasmid

    #104401

    Purpose

    Polycistronic vector for expression of E. coli core RNAP

    Depositor

    Irina Artsimovitch

    Article

    Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

    Insert

    rpoA, B, C, and Z
    

    Use

    E. coli expression vector

    Tags

    encodes a C-terminally His-tagged rpoC which can …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTrc-trGPPS(CO)-LS

    Plasmid

    #50603

    Purpose

    pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

    Depositor

    Jay Keasling

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Inserts

    Limonene Synthase
    ApR
    LacIq
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    truncated codon optimized geranylpyrophosphate sy…

    Promoter

    Trc

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His10 TEV LIC cloning vector (2B-T-10)

    Plasmid

    #78173

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFC902

    Plasmid

    #106904

    Purpose

    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108

    Inserts

    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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