32,812 results

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pTYB2-eIF1A

Plasmid

#37218

Depositor

Jon Lorsch

Article

Acker et al Methods Enzymol. 2007;430:111-45.

Insert

eIF1A

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pKCyR6

Plasmid

#149493

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DLG1_PDZ_1

Plasmid

#103915

Purpose

Protein expression and purification of DLG1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG1 (DLG1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pKESaPar

Plasmid

#211829

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTW411d

Plasmid

#246585

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, NtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYFJ84

Plasmid

#227644

Purpose

Vibrio harveyi acyl-ACP synthetase

Depositor

John Cronan

Article

Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

Insert

Acyl-Acyl carrier protein synthetase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-Leup

Plasmid

#213154

Purpose

Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

Depositor

Jason Crawford

Article

Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

Insert

Leupeptin Protease Inhibitor Pathway

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pQE80l BCB

Plasmid

#196472

Purpose

Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

Depositor

Fei Sun

Article

Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

Insert

BCB

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mECitrine

Plasmid

#105151

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

kanMX6-P3nmt1-mECitrine

Tags

3nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

3nmt1

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mECitrine

Plasmid

#105153

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

Insert

kanMX6-P81nmt1-mECitrine

Tags

81nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

81nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mECitrine

Plasmid

#105152

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

kanMX6-P41nmt1-mECitrine

Tags

41nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

41nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-160

Plasmid

#118540

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pYTK002

Plasmid

#65109

Purpose

Encodes ConLS as a Type 1 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConLS

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Expression

Bacterial

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAD12

Plasmid

#34832

Depositor

Cynthia Kenyon

Article

Dillin et al Science. 2002 Oct 25;298(5594):830-4.

Insert

None

Use

RNAi

Expression

Bacterial

Available Since

June 11, 2012

Availability

Academic Institutions and Nonprofits only
pVEX-CC1

Plasmid

#74170

Purpose

protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Tags

None

Expression

Bacterial

Promoter

Inducible Tac promoter

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
Csy4H29AS50C

Plasmid

#45029

Depositor

Jennifer Doudna

Article

Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

Insert

Csy4H29AS50C

Tags

His6-MBP

Expression

Bacterial

Mutation

H29A mutation to abolish catalytic activity and S…

Promoter

T7

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pBbB2a-GFP

Plasmid

#35343

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX-p40PX(aa1-148)

Plasmid

#19008

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

aa1-148 (PX domains)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pETCON-PTX-S1-220

Plasmid

#67747

Purpose

Pertussis Toxin Subunit 1 Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

PTxS1-220

Expression

Bacterial and Yeast

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSJ155

Plasmid

#27126

Depositor

Alan Finkelstein

Article

Kienker et al J Gen Physiol. 2000 Oct . 116(4):587-98.

Insert

Colicin Ia + Ia immunity protein

Tags

His6

Expression

Bacterial

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pOPINVHH_his_flag

Plasmid

#210404

Purpose

For expression of VHH with C-terminal his and Flag tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

his6-flag

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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PDZK1_PDZ_1

Plasmid

#103927

Purpose

Protein expression and purification of PDZK1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PDZK1_PDZ_4

Plasmid

#103945

Purpose

Protein expression and purification of PDZK1 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PDZK1 (PDZK1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEND-164_Cacnes_pFAST

Plasmid

#225616

Purpose

Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

pFAST

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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6BY9 (EHMT1)

Plasmid

#119956

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

EHMT1 (EHMT1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Omega 2

Plasmid

#118047

Purpose

GoldenBraid2.0 Omega 2 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Alpha2

Plasmid

#118045

Purpose

GoldenBraid2.0 Alpha 2 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Expression

Bacterial, Insect, and Mamm…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

Plasmid

#214950

Purpose

Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
VNp-anti-Lysozyme Fab light chain amino-BiFC fusion

Tags

6xHis, Amino half of venus BIFC fragment, Carboxy…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA528 IST1 (1-366)

Plasmid

#193039

Purpose

Bacterial expression plasmid for full-length IST1 (1-366).

Depositor

Wesley Sundquist

Article

Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

Insert

IST1 (IST1 Human)

Tags

6xHis-Sumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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32,812 results