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32,167 results

Showing: 6991 - 7020 of 32167 results

  1. pET-His6-Sumo-CfaC-MBP

    Plasmid
    #126018
    Purpose
    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)
    Depositor
    Thomas Muir
    Insert
    His6-Sumo-CfaC-MBP
    Tags
    6xHis-SUMO and HA-MBP
    Expression
    Bacterial
    Available Since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126018-plasmid-map-sequence-id-248773

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  2. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid
    #194469
    Purpose
    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Expression
    Bacterial
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194469-plasmid-map-sequence-id-384873

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  8. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  9. DT037A

    Plasmid
    #159398
    Purpose
    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23101-sfGFP
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Promoter
    J23101 (strong) promoter
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159398-plasmid-map-sequence-id-324298

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  23. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid
    #164614
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    stdMCP-stdHalo-RH1
    Use
    Lentiviral
    Tags
    HA tag and NLS
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164614-plasmid-map-sequence-id-325003

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  26. pJB02

    Plasmid
    #111959
    Purpose
    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    T7
    Available Since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111959-plasmid-map-sequence-id-219913

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  30. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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Showing: 6991 - 7020 of 32167 results