32,167 results
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Plasmid#224932PurposeExpresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)DepositorInsertOCaMP
Tags6xHis, RSET peptide, and Xpress tagExpressionBacterialPromoterT7Available SinceJuly 28, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFSKm4-ATF1
Plasmid#221133PurposeFormaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detectionDepositorAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAWO-SUMO-3C_14M_zTDP2
Plasmid#200512PurposeExpression of zebrafish TDP2 recombinant proteinDepositorInsertTDP2 (tdp2b Zebrafish)
TagsHIS-SUMOExpressionBacterialMutationL136N, A139S, C239T, K240R, K309Q, K314L, T315G, …Available SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sender_plasmid2
Plasmid#140693PurposeEncodes inducible expression of RhlI (C4-HSL quorum sensing synthase)DepositorInsertRhlI (rhlI )
TagsssrA degradation tag (ASV)ExpressionBacterialPromoterengineered lac promoter (IPTG inducible)Available SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GDAP2-c005
Plasmid#173110PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pADS
Plasmid#19040DepositorInsertcodon-optimized synthetic amorphadiene synthase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-HISNDHFR
Plasmid#63994PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHis; TEV cleavableExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-E. coli Initiation Factor 3 (IF3)
Plasmid#31340DepositorInsertE. coli Initiation Factor 3
TagsHisExpressionBacterialAvailable SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX3X-RanBP2dFG
Plasmid#53120PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsGSTExpressionBacterialMutationfragment (amino acids 2553-2838)PromotertacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580AAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDFNKI-strepII-3C-LIC-strep
Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-PLproCS-MBP
Plasmid#169195PurposeTo express a PLpro substrate (GST-PLproCS-MBP) in E. coliDepositorInsertGST-PLproCS-MBP
TagsGST-MBP fusion (PLpro-cleavable) and His6 (intern…ExpressionBacterialPromotertac promoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII12Pglu-700uDelta6
Plasmid#13568DepositorInsertFLIPglu F16A Y12S-ECFP-D13R, 6aa linker
TagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBAvailable SinceFeb. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and MammalianPromoterhuman U6/phage T7Available SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDuet TDP-43 G294A
Plasmid#27463DepositorInsertTDP-43 (TARDBP Human)
UseCoexpressionExpressionBacterialMutationGlycine 294 mutated to Alanine (G294A)Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mNeonGreen
Plasmid#191743PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mNeonGreenExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mYPet
Plasmid#103988PurposePlasmid encoding mYPetDepositorInsertmYPet
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, I47L, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXTK025 [Origin_RK2-oriT_PartType1]
Plasmid#229262PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RK2-oriT_PartType1DepositorInsertRK2-oriT Origin
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-indRecA56
Plasmid#102294PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseCRISPRExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrcHUM
Plasmid#19003DepositorInsertgamma-humulene synthase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0323-ss
Plasmid#137067PurposeE. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66700.1
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0323: lacks the BB0323 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9+sgRNA expression vector precursor
Plasmid#171798PurposePICASSO: Library expression vector precursor for paired dCas9+sgRNA expressionDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterpLtetOAvailable SinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJS007
Plasmid#188330PurposePCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::wrmScarlet::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
Tags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only